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. 1978 Aug;5(8):2991–2997. doi: 10.1093/nar/5.8.2991

Substrate dependence of the mechanism of EcoRI endonuclease.

R A Rubin, P Modrich
PMCID: PMC342221  PMID: 211492

Abstract

The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double strand cleavage of SV40 DNA occurs without dissociation of the enzyme in only 75% of the catalytic events. This mechanistic difference presumably reflects sequence differences about the EcoRI sites of these DNA's.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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