Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1978 Aug;5(8):3071–3079. doi: 10.1093/nar/5.8.3071

Excretion of newly synthesized DNA by tonsil lymphocytes.

M Staub, F Antoni
PMCID: PMC342227  PMID: 693326

Abstract

The main part of newly synthesized DNA is preferentially released in vitro both by non-stimulated or phytohemagglutinin-stimulated tonsil lymphocytes. Freshly isolated cells excrete DNA faster than phytohemagglutinin-stimulated ones. The acid-precipitable 3H-thymidine lost can be accounted for in the culture medium as labeled, double stranded DNA isolated on hydroxyapatite column.

Full text

PDF
3071

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson L. C., Häyry P. Generation of T memory cells in one-way mixed lymphocyte culture. I. Selective recovery of activated cells and their reversion to 'secondary' lymphocytes. Scand J Immunol. 1974;3(4):461–469. doi: 10.1111/j.1365-3083.1974.tb01279.x. [DOI] [PubMed] [Google Scholar]
  2. Anker P., Stroun M., Maurice P. A. Spontaneous extracellular synthesis of DNA released by human blood lymphocytes. Cancer Res. 1976 Aug;36(8):2832–2839. [PubMed] [Google Scholar]
  3. Anker P., Stroun M., Maurice P. A. Spontaneous release of DNA by human blood lymphocytes as shown in an in vitro system. Cancer Res. 1975 Sep;35(9):2375–2382. [PubMed] [Google Scholar]
  4. Bernheim J. L., Mendelsohn J., Kelley M. F., Dorian R. Kinetics of cell death and disintegration in human lymphocyte cultures. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2536–2540. doi: 10.1073/pnas.74.6.2536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Demidenko O. E., Tsvetkova S. E. Vykhod DNK iz iader gepatotsitov krys pri obrabotke ikh geparinom. Biull Eksp Biol Med. 1977 May;83(5):551–553. [PubMed] [Google Scholar]
  6. Gatien J. G., Schneeberger E. E., Parkman R., Merler E. Isolation on discontinuous gradients of bovine albumin of a subpopulation of human lymphocytes exhibiting precursor characteristics. Eur J Immunol. 1975 May;5(5):306–312. doi: 10.1002/eji.1830050504. [DOI] [PubMed] [Google Scholar]
  7. Hoessli D. C., Jones A. P., Eisenstadt J. M., Waksman B. H. Studies on DNA release by cultured rat lymphoblasts. Int Arch Allergy Appl Immunol. 1977;54(6):517–528. doi: 10.1159/000231872. [DOI] [PubMed] [Google Scholar]
  8. Izui S., Lambert P. H., Fournié G. J., Türler H., Miescher P. A. Features of systemic lupus erythematosus in mice injected with bacterial lipopolysaccharides: identificantion of circulating DNA and renal localization of DNA-anti-DNA complexes. J Exp Med. 1977 May 1;145(5):1115–1130. doi: 10.1084/jem.145.5.1115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Meinke W., Goldstein D. A., Hall M. R. Rapid isolation of mouse DNA from cells in tissue culture. Anal Biochem. 1974 Mar;58(1):82–88. doi: 10.1016/0003-2697(74)90444-8. [DOI] [PubMed] [Google Scholar]
  11. Meuth M., Aufreiter E., Reichard P. Deoxyribonucleotide pools in mouse-fibroblast cell lines with altered ribonucleotide reductase. Eur J Biochem. 1976 Dec;71(1):39–43. doi: 10.1111/j.1432-1033.1976.tb11087.x. [DOI] [PubMed] [Google Scholar]
  12. Moroni C., Schumann G. Are endogenous C-type viruses involved in the immune system? Nature. 1977 Oct 13;269(5629):600–601. doi: 10.1038/269600a0. [DOI] [PubMed] [Google Scholar]
  13. Rogers J. C., Boldt D., Kornfeld S., Skinner A., Valeri C. R. Excretion of deoxyribonucleic acid by lymphocytes stimulated with phytohemagglutinin or antigen. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1685–1689. doi: 10.1073/pnas.69.7.1685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rogers J. C. Characterization of DNA excreted from phytohemagglutinin-stimulated lymphocytes. J Exp Med. 1976 May 1;143(5):1249–1264. doi: 10.1084/jem.143.5.1249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rogers J. C. Identification of an intracellular precursor to DNA excreted by human lymphocytes. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3211–3215. doi: 10.1073/pnas.73.9.3211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sasvári-Székely M., Vitéz M., Staub M., Antoni F. Determination of UTP and ATP pool sizes in human tonsillar lymphocytes by using Escherichia coli RNA polymerase. Biochim Biophys Acta. 1975 Jul 7;395(3):221–228. doi: 10.1016/0005-2787(75)90192-6. [DOI] [PubMed] [Google Scholar]
  17. Staub M., Antoni F., Sellyei M. DNA synthesis in tonsil lymphocytes. I. Changes in cell population during culture. Biochem Med. 1976 Jun;15(3):246–253. doi: 10.1016/0006-2944(76)90055-7. [DOI] [PubMed] [Google Scholar]
  18. Staub M., Sasvári-Székely M., Spasokukotskaja T., Antoni F., Merétey K. Differences between human lymphocyte subpopulations with respect to the uptake and incorporation of [3H]uridine and ribonucleoside triphosphate pools. Biochem Med. 1978 Apr;19(2):218–230. doi: 10.1016/0006-2944(78)90023-6. [DOI] [PubMed] [Google Scholar]
  19. Tyrsted G., Munch-Petersen B. Early effects of phytohemagglutinin on induction of DNA polymerase, thymidine kinase, deoxyribonucleoside triphosphate pools and DNA synthesis in human lymphocytes. Nucleic Acids Res. 1977 Aug;4(8):2713–2723. doi: 10.1093/nar/4.8.2713. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES