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. 1978 Sep;5(9):3303–3313. doi: 10.1093/nar/5.9.3303

DNA replication in Physarum polycephalum: UV photolysis of maturing 5-bromo-deoxyuridine substituted DNA.

S Funderud, R Andreassen, F Haugli
PMCID: PMC342250  PMID: 309109

Abstract

Combinations of 5-bromodeoxyuridine (BrdUrd) and 3H-deoxyadenosine (3H-DAdo) short pulses were given in the synchronous DNA-replication period of Physarum polycephalum. After a chase period, UV-photolysis products were analyzed on alkaline sucrose gradients. This strategy has allowed the following conclusions. a) at the time of master-initiation of DNA replication, points separated by 1.1-2.2x10(7) daltons of single strand DNA may initiate DNA synthesis. b) among these, only selected groups of replicons actually proceed in DNA replication at this time, while others appear to hold (later temporal sets of replicons). The origins of the ones that proceed in replication are separated from each other by a distance corresponding to 1.1-2.x10(7) daltons. c) regions in actual replication are separated from each other by increasing distances (up to 1.5x10(8) daltons single strand DNA) at later times in S.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Funderud S., Andreassen R., Haugli F. DNA replication in Physarum polycephalum: bidirectional replication of DNA within replicons. Nucleic Acids Res. 1978 Mar;5(3):713–721. doi: 10.1093/nar/5.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Funderud S., Haugli F. DNA replication in Physarum polycephalum: characterization of replication products in vivo. Nucleic Acids Res. 1975 Aug;2(8):1381–1390. doi: 10.1093/nar/2.8.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hutchinson F. The lesions produced by ultraviolet light in DNA containing 5-bromouracil. Q Rev Biophys. 1973 May;6(2):201–246. doi: 10.1017/s0033583500001141. [DOI] [PubMed] [Google Scholar]
  4. McKenna W. G., Masters M. Biochemical evidence for the bidirectional replication of DNA in Escherichia coli. Nature. 1972 Dec 29;240(5383):536–539. doi: 10.1038/240536a0. [DOI] [PubMed] [Google Scholar]
  5. Mohberg J., Rusch H. P. Isolation and DNA content of nuclei of Physarum polycephalum. Exp Cell Res. 1971 Jun;66(2):305–316. doi: 10.1016/0014-4827(71)90682-3. [DOI] [PubMed] [Google Scholar]
  6. Muldoon J. J., Evans T. E., Nygaard O. F., Evans H. H. Control of DNA replication by protein synthesis at defined times during the S period in Physarum polycephalum. Biochim Biophys Acta. 1971 Oct 14;247(2):310–321. doi: 10.1016/0005-2787(71)90679-4. [DOI] [PubMed] [Google Scholar]
  7. Planck S. R., Mueller G. C. Deoxyribonucleic acid chain growth and organization of replicating units in HeLa cells. Biochemistry. 1977 May 3;16(9):1808–1813. doi: 10.1021/bi00628a007. [DOI] [PubMed] [Google Scholar]
  8. Weintraub H. Bi-directional initiation of DNA synthesis in developing chick erythroblasts. Nat New Biol. 1972 Apr 19;236(68):195–197. doi: 10.1038/newbio236195a0. [DOI] [PubMed] [Google Scholar]
  9. Wille J. J., Jr, Kauffman S. A. Premature replication of late S period DNA regions in early S nuclei transferred to late S cytoplasm by fusion in Physarum polycephalum. Biochim Biophys Acta. 1975 Oct 1;407(2):158–173. doi: 10.1016/0005-2787(75)90282-8. [DOI] [PubMed] [Google Scholar]

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