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. 1979 Nov 10;7(5):1121–1130. doi: 10.1093/nar/7.5.1121

A nuclear precursor to human gamma-globin messenger RNA.

M Courtney, R Williamson
PMCID: PMC342290  PMID: 514808

Abstract

RNA isolated from human foetal liver at an erythroid stage of development was transferred from methyl mercury hydroxide agarose gels to diazobenzyloxymethyl paper. A 16S RNA species containing gamma-globin mRNA sequences was identified by hybridisation to 32P-labelled human gamma-globin cDNA recombinant plasmid pHgamma GI. This RNA is found only in the cell nucleus and is polyadenylated. Human globin gene transcription is thus analagous to that in mouse: interventing sequences are transcribed and processed from the pre-mRNA in the nucleus after polyadenylation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bailey J. M., Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. doi: 10.1016/s0003-2697(76)80049-8. [DOI] [PubMed] [Google Scholar]
  4. Basch R. S. Hemoglobin synthesis in short-term cultures of human fetal hematopoietic tissues. Blood. 1972 Apr;39(4):530–541. [PubMed] [Google Scholar]
  5. Bastos R. N., Aviv H. Globin RNA precursor molecules: biosynthesis and process in erythroid cells. Cell. 1977 Jul;11(3):641–650. doi: 10.1016/0092-8674(77)90081-2. [DOI] [PubMed] [Google Scholar]
  6. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Curtis P. J., Weissmann C. Purification of globin messenger RNA from dimethylsulfoxide-induced Friend cells and detection of a putative globin messenger RNA precursor. J Mol Biol. 1976 Oct 5;106(4):1067–1075. doi: 10.1016/0022-2836(76)90353-3. [DOI] [PubMed] [Google Scholar]
  8. Harpold M. M., Dobner P. R., Evans R., Bancroft F. C., Darnell J. E., Jr The synthesis and processing of a nuclear RNA precursor to rat pregrowth hormone messenger RNA. Nucleic Acids Res. 1979 Jul 11;6(9):3133–3144. doi: 10.1093/nar/6.9.3133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jeffreys A. J., Flavell R. A. A physical map of the DNA regions flanking the rabbit beta-globin gene. Cell. 1977 Oct;12(2):429–439. doi: 10.1016/0092-8674(77)90119-2. [DOI] [PubMed] [Google Scholar]
  10. Jost J. P., Pehling G., Ohno T., Cozens P. Identification of a large precursor of vitellogenin mRNA in the liver of estradiol-treated chicks. Nucleic Acids Res. 1978 Dec;5(12):4781–4793. doi: 10.1093/nar/5.12.4781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kinniburgh A. J., Mertz J. E., Ross J. The precursor of mouse beta-globin messenger RNA contains two intervening RNA sequences. Cell. 1978 Jul;14(3):681–693. doi: 10.1016/0092-8674(78)90251-9. [DOI] [PubMed] [Google Scholar]
  12. Kwan S. P., Wood T. G., Lingrel J. B. Purification of a putative precursor of globin messenger RNA from mouse nucleated erythroid cells. Proc Natl Acad Sci U S A. 1977 Jan;74(1):178–182. doi: 10.1073/pnas.74.1.178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lanyon W. G., Ottolenghi S., Williamson R. Human globin gene expression and linkage in bone marrow and fetal liver. Proc Natl Acad Sci U S A. 1975 Jan;72(1):258–262. doi: 10.1073/pnas.72.1.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lehrach H., Diamond D., Wozney J. M., Boedtker H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977 Oct 18;16(21):4743–4751. doi: 10.1021/bi00640a033. [DOI] [PubMed] [Google Scholar]
  15. Lewin B. Units of transcription and translation: the relationship between heterogeneous nuclear RNA and messenger RNA. Cell. 1975 Jan;4(1):11–20. doi: 10.1016/0092-8674(75)90128-2. [DOI] [PubMed] [Google Scholar]
  16. Little P. F., Flavell R. A., Kooter J. M., Annison G., Williamson R. Structure of the human fetal globin gene locus. Nature. 1979 Mar 15;278(5701):227–231. doi: 10.1038/278227a0. [DOI] [PubMed] [Google Scholar]
  17. Little P., Curtis P., Coutelle C., Van den Berg J., Dalgleish R., Malcolm S., Courtney M., Westaway D., Williamson R. Isolation and partial sequence of recombinant plasmids containing human alpha-, beta- and gamma-globin cDNA fragments. Nature. 1978 Jun 22;273(5664):640–643. doi: 10.1038/273640a0. [DOI] [PubMed] [Google Scholar]
  18. Nordstrom J. L., Roop D. R., Tsai M. J., O'Malley B. W. Identification of potential ovomucoid mRNA precursors in chick oviduct nuclei. Nature. 1979 Mar 22;278(5702):328–331. doi: 10.1038/278328a0. [DOI] [PubMed] [Google Scholar]
  19. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  20. Roop D. R., Nordstrom J. L., Tsai S. Y., Tsai M. J., O'Malley B. W. Transcription of structural and intervening sequences in the ovalbumin gene and identification of potential ovalbumin mRNA precursors. Cell. 1978 Oct;15(2):671–685. doi: 10.1016/0092-8674(78)90035-1. [DOI] [PubMed] [Google Scholar]
  21. Ross J. A precursor of globin messenger RNA. J Mol Biol. 1976 Sep 15;106(2):403–420. doi: 10.1016/0022-2836(76)90093-0. [DOI] [PubMed] [Google Scholar]
  22. Ross J., Knecht D. A. Precursors of alpha and beta globin messenger RNAs. J Mol Biol. 1978 Feb 15;119(1):1–20. doi: 10.1016/0022-2836(78)90266-8. [DOI] [PubMed] [Google Scholar]
  23. Schibler U., Marcu K. B., Perry R. P. The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells. Cell. 1978 Dec;15(4):1495–1509. doi: 10.1016/0092-8674(78)90072-7. [DOI] [PubMed] [Google Scholar]
  24. Smithies O., Blechl A. E., Denniston-Thompson K., Newell N., Richards J. E., Slightom J. L., Tucker P. W., Blattner F. R. Cloning human fetal gamma globin and mouse alpha-type globin DNA: characterization and partial sequencing. Science. 1978 Dec 22;202(4374):1284–1289. doi: 10.1126/science.725604. [DOI] [PubMed] [Google Scholar]
  25. Strair R. K., Skoultchi A. I., Shafritz D. A. A characterization of globin mRNA sequences in the nucleus of duck immature red blood cells. Cell. 1977 Sep;12(1):133–141. doi: 10.1016/0092-8674(77)90191-x. [DOI] [PubMed] [Google Scholar]
  26. Tilghman S. M., Curtis P. J., Tiemeier D. C., Leder P., Weissmann C. The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1309–1313. doi: 10.1073/pnas.75.3.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tilghman S. M., Tiemeier D. C., Seidman J. G., Peterlin B. M., Sullivan M., Maizel J. V., Leder P. Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene. Proc Natl Acad Sci U S A. 1978 Feb;75(2):725–729. doi: 10.1073/pnas.75.2.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wellauer P. K., Dawid I. B. Secondary structure maps of RNA: processing of HeLa ribosomal RNA. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2827–2831. doi: 10.1073/pnas.70.10.2827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wood W. G., Weatherall D. J. Haemoglobin synthesis during human foetal development. Nature. 1973 Jul 20;244(5412):162–165. doi: 10.1038/244162a0. [DOI] [PubMed] [Google Scholar]
  31. Wynter C. V., Ioannou P., Mathias A. P. The effect of convulsions induced by flurothyl on ribonucleic acid synthesis in rat cerebral cortex during the recovery phase. Biochem J. 1975 Dec;152(3):449–467. doi: 10.1042/bj1520449. [DOI] [PMC free article] [PubMed] [Google Scholar]

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