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. 1979 Nov 24;7(6):1675–1686. doi: 10.1093/nar/7.6.1675

Evidences for the function of DNA polymerase-beta in unscheduled DNA synthesis.

E Wawra, I Dolejs
PMCID: PMC342336  PMID: 503866

Abstract

The activities of DNA polymerase-alpha and -beta isolated from pig spleen were determined at different temperatures and in the presence of different concentrations of inhibitors. The results were compared with parallel estimations of replicative DNA synthesis and UV-induced repair synthesis in spleen cells. In respect to pCMB and aCTP, polymerase-alpha is more sensitive than polymerase-beta and similarly is replication more sensitive than repair. Repair synthesis and the activity of polymerase-beta decreases at temperatures higher than 40 degrees C whereas both replication and the activity of polymerase-alpha are greatly stimulated at elevated temperatures with optima of 45 degrees C (polymerase-alpha) and 41 degrees C (replication). The results favour the hypothesis that polymerase-beta is involved in repair synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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