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. 1979 Dec 11;7(7):1787–1798. doi: 10.1093/nar/7.7.1787

Isolation of a genomal clone containing chicken histone genes.

R P Harvey, J R Wells
PMCID: PMC342348  PMID: 161399

Abstract

We have used enriched chicken histone cDNA to select genomal clones from a chicken library. Because the cDNA probe also contained other sequences, a further screening of positive plagues with negative probes eliminated most non-histone gene clones. One 'positively-selected' genomal clone, lambda CH-01, hybridised with cloned sea-urchin histone genes and also detected histone genes in EcoRI-digested genomal sea-urchin DNA. Limited DNA sequencing of HaeIII fragments identified two sequences within the coding region of chicken histone H2A. A third fragment predicted an amino acid sequence with strong homology to an H1 histone sequence.

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Selected References

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