Table 1.
Δ5 Desaturase activity of EgΔ5D with HxGG mutations
| Gene name | Sequence of HPGG motif | Δ5 Conversion (%) | % of wild type EgΔ5D |
|---|---|---|---|
| EgΔ5D | HPGG | 24.2 | 100 |
| EgΔ5D-34A | HaGG | 22.7 | 93.5 |
| EgΔ5D-34C | HcGG | 22.6 | 93.2 |
| EgΔ5D-34D | HdGG | 12.5 | 51.6 |
| EgΔ5D-34E | HeGG | 14.7 | 60.5 |
| EgΔ5D-34F | HfGG | 17.9 | 73.9 |
| EgΔ5D-34G | HgGG | 23.8 | 98.2 |
| EgΔ5D-34H | HhGG | 21.3 | 87.8 |
| EgΔ5D-34I | HiGG | 18.3 | 75.4 |
| EgΔ5D-34K | HkGG | 22.7 | 93.8 |
| EgΔ5D-34L | HlGG | 17.0 | 70.0 |
| EgΔ5D-34M | HmGG | 19.0 | 78.4 |
| EgΔ5D-34N | HnGG | 19.8 | 81.5 |
| EgΔ5D-34Q | HqGG | 19.8 | 81.7 |
| EgΔ5D-34R | HrGG | 19.4 | 79.9 |
| EgΔ5D-34S | HsGG | 20.4 | 84.1 |
| EgΔ5D-34T | HtGG | 19.6 | 80.7 |
| EgΔ5D-34V | HvGG | 20.2 | 83.4 |
| EgΔ5D-34W | HwGG | 22.2 | 91.7 |
| EgΔ5D-34Y | HyGG | 17.7 | 73.1 |
Average of 6 samples for each construct containing different mutations