Table 3.
Δ5 Desaturase activity of EgΔ5D with HDxSH mutations
| Gene name | Sequence of HDASH motif | Δ5 Conversion (%) | % of wild type EgΔ5D |
|---|---|---|---|
| EgΔ5D | HDASH | 24.8 | 100 |
| EgΔ5D-157C | HDcSH | 10.7 | 43.1 |
| EgΔ5D-157D | HDdSH | 1.0 | 4.0 |
| EgΔ5D-157E | HDeSH | 0.9 | 3.6 |
| EgΔ5D-157F | HDfSH | 1.0 | 4.0 |
| EgΔ5D-157G | HDgSH | 23.8 | 96 |
| EgΔ5D-157H | HDhSH | 1.0 | 4.0 |
| EgΔ5D-157I | HDiSH | 0.9 | 3.6 |
| EgΔ5D-157K | HDkSH | 1.0 | 4.0 |
| EgΔ5D-157L | HDlSH | 1.1 | 4.4 |
| EgΔ5D-157M | HDmSH | 1.0 | 4.0 |
| EgΔ5D-157N | HDnSH | 1.1 | 4.4 |
| EgΔ5D-157P | HDpSH | 2.3 | 9.3 |
| EgΔ5D-157Q | HDqSH | 0.6 | 2.4 |
| EgΔ5D-157R | HDrSH | 0.8 | 3.2 |
| EgΔ5D-157S | HDsSH | 23.3 | 94 |
| EgΔ5D-157T | HDtSH | 1.0 | 4.0 |
| EgΔ5D-157V | HDvSH | 0.3 | 1.2 |
| EgΔ5D-157W | HDwSH | 0.9 | 3.6 |
| EgΔ5D-157Y | HDySH | 0.7 | 2.8 |
Average of 3 samples for each construct containing different mutations