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. 1979 Dec 20;7(8):2165–2176. doi: 10.1093/nar/7.8.2165

Isolation of messenger RNA coding for the "fast" protein of embryonic chick feathers.

B C Powell, G E Rogers
PMCID: PMC342377  PMID: 523317

Abstract

The messenger RNA coding for the "Fast" protein of embryonic chick feathers has been purified from the overwhelming relative amounts of keratin mRNA which are present in the developing feathers. The "Fast" protein mRNA represents about 4-8% of the total mRNA population of the feather. Despite differences between the size of the "Fast" proteins and the keratins the two mRNA species are very similar in molecular weight as judged by electrophoresis under denaturing conditions. However, by electrophoresis in 8 M urea gels at 55 degrees C, the "Fast" protein mRNA could be separated from keratin mRNA, presumably reflecting differences in messenger RNA secondary structure.

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Selected References

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  1. Baralle F. Complete nucleotide sequence of the 5' noncoding region of human alpha-and beta-globin mRNA. Cell. 1977 Dec;12(4):1085–1095. doi: 10.1016/0092-8674(77)90171-4. [DOI] [PubMed] [Google Scholar]
  2. Berns A. J., Strous G. J., Bloemendal H. Heterologous in vitro synthesis of lens -crystallin polypeptide. Nat New Biol. 1972 Mar 1;236(61):7–9. doi: 10.1038/newbio236007a0. [DOI] [PubMed] [Google Scholar]
  3. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  4. Bryan R. N., Cutter G. A., Hayashi M. Separate mRNAs code for tubulin subunits. Nature. 1978 Mar 2;272(5648):81–83. doi: 10.1038/272081a0. [DOI] [PubMed] [Google Scholar]
  5. Cleveland D. W., Kirschner M. W., Cowan N. J. Isolation of separate mRNAs for alpha- and beta-tubulin and characterization of the corresponding in vitro translation products. Cell. 1978 Nov;15(3):1021–1031. doi: 10.1016/0092-8674(78)90286-6. [DOI] [PubMed] [Google Scholar]
  6. Kemp D. J., Dyer P. Y., Rogers G. E. Keratin synthesis during development of the embryonic chick feather. J Cell Biol. 1974 Jul;62(1):114–131. doi: 10.1083/jcb.62.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kemp D. J., Partington G. A., Rogers G. E. Isolation and molecular weight of pure feather keratin mRNA. Biochem Biophys Res Commun. 1974 Oct 8;60(3):1006–1014. doi: 10.1016/0006-291x(74)90413-6. [DOI] [PubMed] [Google Scholar]
  8. Kemp D. J., Schwinghamer M. W., Rogers G. E. Translation of pure feather keratin mRNA in a wheat embryo cell-free system. Mol Biol Rep. 1974 Dec;1(8):441–446. doi: 10.1007/BF00360669. [DOI] [PubMed] [Google Scholar]
  9. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  10. Lehrach H., Diamond D., Wozney J. M., Boedtker H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977 Oct 18;16(21):4743–4751. doi: 10.1021/bi00640a033. [DOI] [PubMed] [Google Scholar]
  11. Lizardi P. M., Williamson R., Brown D. D. The size of fibroin messenger RNA and its polyadenylic acid content. Cell. 1975 Mar;4(3):199–205. doi: 10.1016/0092-8674(75)90168-3. [DOI] [PubMed] [Google Scholar]
  12. Marcu K., Dudock B. Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acids Res. 1974 Nov;1(11):1385–1397. doi: 10.1093/nar/1.11.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McReynolds L., O'Malley B. W., Nisbet A. D., Fothergill J. E., Givol D., Fields S., Robertson M., Brownlee G. G. Sequence of chicken ovalbumin mRNA. Nature. 1978 Jun 29;273(5665):723–728. doi: 10.1038/273723a0. [DOI] [PubMed] [Google Scholar]
  14. O'Donnell I. J. A search for a simple keratin--fractionation and peptide mapping of proteins from feather keratins. Aust J Biol Sci. 1973 Apr;26(2):401–413. doi: 10.1071/bi9730401. [DOI] [PubMed] [Google Scholar]
  15. Osterburg H. H., Allen J. K., Finch C. E. The use of ammonium acetate in the precipitation of ribonucleic acid. Biochem J. 1975 May;147(2):367–368. doi: 10.1042/bj1470367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Partington G. A., Kemp D. J., Rogers G. E. Isolation of feather keratin mRNA and its translation in a rabbit reticulocyte cell-free system. Nat New Biol. 1973 Nov 14;246(150):33–36. doi: 10.1038/newbio246033a0. [DOI] [PubMed] [Google Scholar]
  17. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  18. Powell B. C., Kemp D. J., Partington G. A., Gibbs P. E., Rogers G. E. Control of feather keratin synthesis by the availability of keratin mRNA. Biochem Biophys Res Commun. 1976 Feb 23;68(4):1263–1271. doi: 10.1016/0006-291x(76)90333-8. [DOI] [PubMed] [Google Scholar]
  19. Reijnders L., Sloof P., Sival J., Borst P. Gel electrophoresis of RNA under denaturing conditions. Biochim Biophys Acta. 1973 Oct 26;324(3):320–333. doi: 10.1016/0005-2787(73)90278-5. [DOI] [PubMed] [Google Scholar]
  20. Spohr G., Mirault M. E., Imaizumi T., Scherrer K. Molecular-weight determination of animal-cell RNA by electrophoresis in formamide under fully denaturing conditions on exponential polyacrylamide gels. Eur J Biochem. 1976 Feb 16;62(2):313–322. doi: 10.1111/j.1432-1033.1976.tb10163.x. [DOI] [PubMed] [Google Scholar]
  21. Weeds A. G. Light chains from slow-twitch muscle myosin. Eur J Biochem. 1976 Jun 15;66(1):157–173. doi: 10.1111/j.1432-1033.1976.tb10436.x. [DOI] [PubMed] [Google Scholar]

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