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. 1979 Dec 20;7(8):2275–2288. doi: 10.1093/nar/7.8.2275

Regions of the polyoma genome coding for T antigens.

T Hunter, M A Hutchinson, W Eckhart, T Friedmann, A Esty, P LaPorte, P Deininger
PMCID: PMC342384  PMID: 230465

Abstract

The early region of the polyoma genome encodes three T antigens. We have analyzed the organization of the coding regions for the T antigens, using the nucleotide sequence of polyoma DNA and peptides derived from purified, radio-labeled T antigens, separated by two-dimensional electrophoresis and chromatography. We compared the peptides, predicted from the nucleotide sequence of the DNA, with those derived from the purified T antigens. We also compared chemically synthesized peptides, predicted from the DNA sequence, with observed peptides. The results show that the three polyoma T antigens are encoded in overlapping regions of the viral DNA, translated, in part, in two different reading frames.

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Selected References

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