Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1979 Dec 20;7(8):2289–2301. doi: 10.1093/nar/7.8.2289

The nucleotide sequence preceding and including the beginning of the ilvE gene of the ilvGEDA operon of Escherichia coli K12.

R P Lawther, B Nichols, G Zurawski, G W Hatfield
PMCID: PMC342385  PMID: 392469

Abstract

The DNA sequence of the 570 base pairs that precedes and includes the beginning of the ilvE gene shows no evidence of a leader-attenuator region. Instead, the sequence shows that the ilvE gene is preceded by another structural gene, presumably the normally cryptic ilvG gene. In vitro transcription of the two plasmids (pLC26-3 and pRL5) containing the regulatory region of the ilvEDA operon results in the formation of two "leader" RNAs. These results are consistent with the suggestion that the ilvEDA operon is regulated by an attenuator mechanism and that the attenuator region lies before the ilvG gene.

Full text

PDF

Images in this article

2295Image
on p.2295
2298Image
on p.2298

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams C. W., Lawther R. P., Hatfield G. W. The ilvEDA operon of Escherichia coli K12 encodes only one valine-alpha-ketoglutarate transaminase activity. Biochem Biophys Res Commun. 1979 Jul 27;89(2):650–658. doi: 10.1016/0006-291x(79)90679-x. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baez M., Patin D. W., Calhoun D. H. Deletion mapping of the ilvGOEDAC genes of Escherichia coli K-12. Mol Gen Genet. 1979 Feb 1;169(3):289–297. doi: 10.1007/BF00382275. [DOI] [PubMed] [Google Scholar]
  4. Bertrand K., Korn L., Lee F., Platt T., Squires C. L., Squires C., Yanofsky C. New features of the regulation of the tryptophan operon. Science. 1975 Jul 4;189(4196):22–26. doi: 10.1126/science.1094538. [DOI] [PubMed] [Google Scholar]
  5. Childs G. J., Ohtsubo H., Ohtsubo E., Sonnenberg F., Freundlich M. Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes. J Mol Biol. 1977 Nov 25;117(1):175–193. doi: 10.1016/0022-2836(77)90030-4. [DOI] [PubMed] [Google Scholar]
  6. Cohen B. M., Jones E. W. New Map Location of ilvO in ESCHERICHIA COLI. Genetics. 1976 Jun;83(2):201–225. doi: 10.1093/genetics/83.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. De Felice M., Squires C., Levinthal M., Guardiola J., Lamberti A., Iaccarino M. Growth inhibition of Escherichia coli K-12 by L-valine: a consequence of a regulatory pattern. Mol Gen Genet. 1977 Nov 4;156(1):1–7. doi: 10.1007/BF00272245. [DOI] [PubMed] [Google Scholar]
  8. Favre R., Wiater A., Puppo S., Iaccarino M. Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12. Mol Gen Genet. 1976 Feb 2;143(3):243–252. doi: 10.1007/BF00269400. [DOI] [PubMed] [Google Scholar]
  9. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  10. Lawther R. P., Hatfield G. W. A site of action for tRNA mediated regulation of the ilvOEDA operon of Escherichia coli K12. Mol Gen Genet. 1978 Nov 29;167(2):227–234. doi: 10.1007/BF00266916. [DOI] [PubMed] [Google Scholar]
  11. Lee F., Yanofsky C. Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4365–4369. doi: 10.1073/pnas.74.10.4365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCorkle G. M., Leathers T. D., Umbarger H. E. Physical organization of the ilvEDAC genes of Escherichia coli strain K-12. Proc Natl Acad Sci U S A. 1978 Jan;75(1):89–93. doi: 10.1073/pnas.75.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES. J Bacteriol. 1965 Mar;89:661–664. doi: 10.1128/jb.89.3.661-664.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith J. M., Smith F. J., Umbarger H. E. Mutations affecting the formation of acetohydroxy acid synthase II in Escherichia coli K-12. Mol Gen Genet. 1979 Feb 1;169(3):299–314. doi: 10.1007/BF00382276. [DOI] [PubMed] [Google Scholar]
  16. Smith J. M., Smolin D. E., Umbarger H. E. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12. Mol Gen Genet. 1976 Oct 18;148(2):111–124. doi: 10.1007/BF00268374. [DOI] [PubMed] [Google Scholar]
  17. Sutcliffe J. G. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 1978 Aug;5(8):2721–2728. doi: 10.1093/nar/5.8.2721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Umbarger H. E. Amino acid biosynthesis and its regulation. Annu Rev Biochem. 1978;47:532–606. doi: 10.1146/annurev.bi.47.070178.002533. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES