Abstract
Severe degradation of high molecular weight RNA was shown to occur during incubation with commercially purified DNase. Most of the RNase activity could be removed by passage of the DNase through a column of agarose-coupled amino phenylphosphoryl-uridine-2' (3')-phosphate. Incubation with the treated DNase caused only minimal alteration of the sedimentation pattern of high molecular weight nuclear RNA, determined under partially denturing conditions. No impairment of DNase activity was detected.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bantle J. A., Maxwell I. H., Hahn W. E. Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA. Anal Biochem. 1976 May 7;72:413–427. doi: 10.1016/0003-2697(76)90549-2. [DOI] [PubMed] [Google Scholar]
- Kirby K. S., Cook E. A. Isolation of deoxyribonucleic acid from mammalian tissues. Biochem J. 1967 Jul;104(1):254–257. doi: 10.1042/bj1040254. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kirby K. S., Fox-Carter E., Guest M. Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria. Biochem J. 1967 Jul;104(1):258–262. doi: 10.1042/bj1040258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxwell I. H. Intracellular distribution and sedimentation properties of virus-specific RNA in two clones of BHK cells transformed by polyoma virus. J Virol. 1976 May;18(2):461–472. doi: 10.1128/jvi.18.2.461-472.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilchek M., Gorecki M. Affinity chromatography of bovine pancreatic ribonuclease A. Eur J Biochem. 1969 Dec;11(3):491–494. doi: 10.1111/j.1432-1033.1969.tb00799.x. [DOI] [PubMed] [Google Scholar]
- Zimmerman S. B., Sandeen D. The ribonuclease activity of crystallized pancreatic deoxyribonuclease. Anal Biochem. 1966 Feb;14(2):269–277. doi: 10.1016/0003-2697(66)90137-0. [DOI] [PubMed] [Google Scholar]