Abstract
tRNAAsp from rabbit liver, rat liver and rat ascites hepatoma was readily isolated by concanavalin A-Sepharose (Con A-Sepharose) affinity column chromatography. tRNATyr from these sources was extensively purified by Ricinus communis lectin-Sepharose column chromatography. These results, together with the chromatographic behaviour of four tRNAs (tRNATyr, tRNAHis, tRNAAsn and tRNAAsp) on acetylated DBAE-cellulose column chromatography suggested that tRNAAsp contains a Q nucleoside species having a mannose moiety while tRNATyr contains Q nucleoside with galactose. The sugars attached in 4-position of cyclopentene diol in the Q molecule are therefore not present at random in the four tRNAs, but present only in each specific tRNA. This is the first case which shows that plant agglutinin interacts with nucleic Acid as well as polysaccharide and glycoproteins.
Full text
PDF![415](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48b8/342442/f715970b05e4/nar00475-0145.png)
![416](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48b8/342442/ae1a700affe4/nar00475-0146.png)
![417](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48b8/342442/a1061bc89612/nar00475-0147.png)
![418](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48b8/342442/33b87120387a/nar00475-0148.png)
![419](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48b8/342442/c735397ccdb1/nar00475-0149.png)
![420](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48b8/342442/3481e098809e/nar00475-0150.png)
![421](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48b8/342442/dbb951b0e621/nar00475-0151.png)
![422](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48b8/342442/9563af7a0f45/nar00475-0152.png)
![423](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48b8/342442/0ab07565df08/nar00475-0153.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GOLDSTEIN I. J., HOLLERMAN C. E., MERRICK J. M. PROTEIN-CARBOHYDRATE INTERACTION. I. THE INTERACTION OF POLYSACCHARIDES WITH CONCANAVALIN A. Biochim Biophys Acta. 1965 Jan 4;97:68–76. doi: 10.1016/0304-4165(65)90270-9. [DOI] [PubMed] [Google Scholar]
- Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harada F., Nishimura S. Possible anticodon sequences of tRNA His , tRNA Asm , and tRNA Asp from Escherichia coli B. Universal presence of nucleoside Q in the first postion of the anticondons of these transfer ribonucleic acids. Biochemistry. 1972 Jan 18;11(2):301–308. doi: 10.1021/bi00752a024. [DOI] [PubMed] [Google Scholar]
- Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. J Biol Chem. 1973 Jul 25;248(14):5024–5032. [PubMed] [Google Scholar]
- Kasai H., Kuchino Y., Nihei K., Nishimura S. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's. Nucleic Acids Res. 1975 Oct;2(10):1931–1939. doi: 10.1093/nar/2.10.1931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kasai H., Nakanishi K., Macfarlane R. D., Torgerson D. F., Ohashi Z., McCloskey J. A., Gross H. J., Nishimura S. Letter: The structure of Q* nucleoside isolated from rabbit liver transfer ribonucleic acid. J Am Chem Soc. 1976 Aug 4;98(16):5044–5046. doi: 10.1021/ja00432a071. [DOI] [PubMed] [Google Scholar]
- Kasai H., Oashi Z., Harada F., Nishimura S., Oppenheimer N. J., Crain P. F., Liehr J. G., von Minden D. L., McCloskey J. A. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine. Biochemistry. 1975 Sep 23;14(19):4198–4208. doi: 10.1021/bi00690a008. [DOI] [PubMed] [Google Scholar]
- Lis H., Sharon N. The biochemistry of plant lectins (phytohemagglutinins). Annu Rev Biochem. 1973;42(0):541–574. doi: 10.1146/annurev.bi.42.070173.002545. [DOI] [PubMed] [Google Scholar]
- McCutchan T. F., Gilham P. T., Söll D. An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose. Nucleic Acids Res. 1975 Jun;2(6):853–864. doi: 10.1093/nar/2.6.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mirzabekov A. D., Griffin B. E. 5 s RNA conformation. Studies of its partial T 1 ribonuclease digestion by gel electrophoresis and two-dimensional thin-layer chromatography. J Mol Biol. 1972 Dec 30;72(3):633–643. doi: 10.1016/0022-2836(72)90181-7. [DOI] [PubMed] [Google Scholar]
- Nishimura S., Weinstein I. B. Fractionation of rat liver transfer ribonucleic acid. Isolation of tyrosine, valine, serine, and phenylalanine transfer ribonucleic acids and their coding properties. Biochemistry. 1969 Mar;8(3):832–842. doi: 10.1021/bi00831a011. [DOI] [PubMed] [Google Scholar]
- Okada N., Harada F., Nishimura S. Specific replacement of Q base in the anticodon of tRNA by guanine catalyzed by a cell-free extract of rabbit reticulocytes. Nucleic Acids Res. 1976 Oct;3(10):2593–2603. doi: 10.1093/nar/3.10.2593. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simsek M., Ziegenmeyer J., Heckman J., Rajbhandary U. L. Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1041–1045. doi: 10.1073/pnas.70.4.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Székely M., Sanger F. Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids. J Mol Biol. 1969 Aug 14;43(3):607–617. doi: 10.1016/0022-2836(69)90362-3. [DOI] [PubMed] [Google Scholar]
- White B. N., Tener G. M. Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus. J Mol Biol. 1973 Mar 15;74(4):635–651. doi: 10.1016/0022-2836(73)90054-5. [DOI] [PubMed] [Google Scholar]