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. 1977 Mar;4(3):567–572. doi: 10.1093/nar/4.3.567

Lac repressor purification without inactivation of DNA binding activity.

J M Rosenberg, O B Khallai, M L Kopka, R E Dickerson, A D Riggs
PMCID: PMC342462  PMID: 866182

Abstract

A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 +/- 10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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