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. 1977 Apr;4(4):853–866. doi: 10.1093/nar/4.4.853

Salt-induced structural changes in nucleosomes.

C G Sahasrabuddhe, G F Saunders
PMCID: PMC342489  PMID: 866195

Abstract

Nucleosomes and oligonucleosomes were prepared by digestion of human placental nuclei with staphlococcal nuclease and fractionated by gel filtration chromatography. The effect of increasing salt on the structure of nucleosomes was examined in the presence and absence of 10 mM MgCl2. Nucleosomes and oligonucleosomes are insoluble over a broad range of salt concentration. Nucleosomes are insoluble in larger than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 allowing analyses of changes in nucleosomal DNA by C.D. spectroscopy. Nucleosomes are insoluble in less than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 as demonstrated by turbidity measurements. We conclude that the insolubility of nucleosomes accompanies salt-induced structural changes possibly due to individual particle condensation. As the salt concentration is increased the nucleosomes condense and then relax at higher salt concentrations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axel R., Cedar H., Felsenfield G. The structure of the globin genes in chromatin. Biochemistry. 1975 Jun 3;14(11):2489–2495. doi: 10.1021/bi00682a031. [DOI] [PubMed] [Google Scholar]
  2. Axel R., Melchior W., Jr, Sollner-Webb B., Felsenfeld G. Specific sites of interaction between histones and DNA in chromatin. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4101–4105. doi: 10.1073/pnas.71.10.4101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bakayev V. V., Melnickov A. A., Osicka V. D., Varshausky A. J. Studies on chromatin. II. Isolation and characterization of chromatin subunits. Nucleic Acids Res. 1975 Aug;2(8):1401–1419. doi: 10.1093/nar/2.8.1401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark R. J., Felsenfeld G. Structure of chromatin. Nat New Biol. 1971 Jan 27;229(4):101–106. doi: 10.1038/newbio229101a0. [DOI] [PubMed] [Google Scholar]
  5. Compton J. L., Bellard M., Chambon P. Biochemical evidence of variability in the DNA repeat length in the chromatin of higher eukaryotes. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4382–4386. doi: 10.1073/pnas.73.12.4382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. D'Anna J. A., Jr, Isenberg I. A histone cross-complexing pattern. Biochemistry. 1974 Nov 19;13(24):4992–4997. doi: 10.1021/bi00721a019. [DOI] [PubMed] [Google Scholar]
  7. D'Anna J. A., Jr, Isenberg I. Interactions of histone LAK (f2a2) with histones KAS (f2b) and GRK (f2a1). Biochemistry. 1974 May 7;13(10):2098–2104. doi: 10.1021/bi00707a016. [DOI] [PubMed] [Google Scholar]
  8. Hanlon S., Johnson R. S., Wolf B., Chan A. Mixed conformations of deoxyribonucleic acid in chromatin: a preliminary report. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3263–3267. doi: 10.1073/pnas.69.11.3263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hewish D. R., Burgoyne L. A. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochem Biophys Res Commun. 1973 May 15;52(2):504–510. doi: 10.1016/0006-291x(73)90740-7. [DOI] [PubMed] [Google Scholar]
  10. Jirgensons B., Hnilica L. S. The conformational changes of calf-thymus histone fractions as determined by the optical rotary dispersion. Biochim Biophys Acta. 1965 Sep 27;109(1):241–249. doi: 10.1016/0926-6585(65)90108-1. [DOI] [PubMed] [Google Scholar]
  11. Johnson R. S., Chan A., Hanlon S. Mixed conformations of deoxyribonucleic acid in intact chromatin isolated by various preparative methods. Biochemistry. 1972 Nov 7;11(23):4347–4358. doi: 10.1021/bi00773a023. [DOI] [PubMed] [Google Scholar]
  12. Kirby K. S., Cook E. A. Isolation of deoxyribonucleic acid from mammalian tissues. Biochem J. 1967 Jul;104(1):254–257. doi: 10.1042/bj1040254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kornberg R. D. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. doi: 10.1126/science.184.4139.868. [DOI] [PubMed] [Google Scholar]
  14. Lacy E., Axel R. Analysis of DNA of isolated chromatin subunits. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3978–3982. doi: 10.1073/pnas.72.10.3978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Li H. J., Wickett R., Craig A. M., Isenberg I. Conformational changes in histone IV. Biopolymers. 1972 Feb;11(2):375–397. doi: 10.1002/bip.1972.360110206. [DOI] [PubMed] [Google Scholar]
  16. Mandel R., Fasman G. D. Chromatin and nucleosome structure. Nucleic Acids Res. 1976 Aug;3(8):1839–1855. doi: 10.1093/nar/3.8.1839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Marushige K., Bonner J. Template properties of liver chromatin. J Mol Biol. 1966 Jan;15(1):160–174. doi: 10.1016/s0022-2836(66)80218-8. [DOI] [PubMed] [Google Scholar]
  18. Noll M. Subunit structure of chromatin. Nature. 1974 Sep 20;251(5472):249–251. doi: 10.1038/251249a0. [DOI] [PubMed] [Google Scholar]
  19. Olins A. L., Olins D. E. Spheroid chromatin units (v bodies). Science. 1974 Jan 25;183(4122):330–332. doi: 10.1126/science.183.4122.330. [DOI] [PubMed] [Google Scholar]
  20. Oosterhof D. K., Hozier J. C., Rill R. L. Nucleas action on chromatin: evidence for discrete, repeated nucleoprotein units along chromatin fibrils. Proc Natl Acad Sci U S A. 1975 Feb;72(2):633–637. doi: 10.1073/pnas.72.2.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Oudet P., Gross-Bellard M., Chambon P. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975 Apr;4(4):281–300. doi: 10.1016/0092-8674(75)90149-x. [DOI] [PubMed] [Google Scholar]
  22. Pardon J. F., Worcester D. L., Wooley J. C., Tatchell K., Van Holde K. E., Richards B. M. Low-angle neutron scattering from chromatin subunit particles. Nucleic Acids Res. 1975 Nov;2(11):2163–2176. doi: 10.1093/nar/2.11.2163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Polisky B., McCarthy B. Location of histones on simian virus 40 DNA. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2895–2899. doi: 10.1073/pnas.72.8.2895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rill R., Van Holde K. E. Properties of nuclease-resistant fragments of calf thymus chromatin. J Biol Chem. 1973 Feb 10;248(3):1080–1083. [PubMed] [Google Scholar]
  25. Sahasrabuddhe C. G., Van Holde K. E. The effect of trypsin on nuclease-resistant chromatin fragments. J Biol Chem. 1974 Jan 10;249(1):152–156. [PubMed] [Google Scholar]
  26. Shaw B. R., Corden J. L., Sahasrabuddhe C. G., Van Holde K. E. Chromatographic separation of chromatin subunits. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1193–1198. doi: 10.1016/s0006-291x(74)80410-9. [DOI] [PubMed] [Google Scholar]
  27. Shih T. Y., Fasman G. D. Conformation of deoxyribonucleic acid in chromatin: a circular dichroism study. J Mol Biol. 1970 Aug 28;52(1):125–129. doi: 10.1016/0022-2836(70)90182-8. [DOI] [PubMed] [Google Scholar]
  28. Simpson R. T., Sober H. A. Circular dichroism of calf liver nucleohistone. Biochemistry. 1970 Aug 4;9(16):3103–3109. doi: 10.1021/bi00818a001. [DOI] [PubMed] [Google Scholar]
  29. Simpson R. T., Whitlock J. P., Jr Chemical evidence that chromatin DNA exists as 160 base pair beads interspersed with 40 base pair bridges. Nucleic Acids Res. 1976 Jan;3(1):117–127. doi: 10.1093/nar/3.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Solage A., Cedar H. RNA chain elongation on a chromatin template. Nucleic Acids Res. 1976 Sep;3(9):2223–2231. doi: 10.1093/nar/3.9.2223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Staynov D. Z. Thermal denaturation profiles and the structure of chromatin. Nature. 1976 Dec 9;264(5586):522–525. doi: 10.1038/264522a0. [DOI] [PubMed] [Google Scholar]
  32. Steinmetz M., Streeck R. E., Zachau H. G. Nucleosome formation abolishes base-specific binding of histones. Nature. 1975 Dec 4;258(5534):447–450. doi: 10.1038/258447a0. [DOI] [PubMed] [Google Scholar]
  33. Tien Kuo M., Sahasrabuddhe C. G., Saunders G. F. Presence of messenger specifying sequences in the DNA of chromatin subunits. Proc Natl Acad Sci U S A. 1976 May;73(5):1572–1575. doi: 10.1073/pnas.73.5.1572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Tunis-Schneider M. J., Maestre M. F. Circular dichroism spectra of oriented and unoriented deoxyribonucleic acid films--a preliminary study. J Mol Biol. 1970 Sep 28;52(3):521–541. doi: 10.1016/0022-2836(70)90417-1. [DOI] [PubMed] [Google Scholar]
  35. Van Holde K. E., Sahasrabuddhe C. G., Shaw B. R. A model for particulate structure in chromatin. Nucleic Acids Res. 1974 Nov;1(11):1579–1586. doi: 10.1093/nar/1.11.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Yarbrough L. R., Hurwitz J. The reversible denaturation of deoxyribonucleic acid-dependent ribonucleic acid polymerase of Escherichia coli. J Biol Chem. 1974 Sep 10;249(17):5394–5399. [PubMed] [Google Scholar]
  37. van Bruggen E. F., Arnberg A. C., van Holde K. E., Sahasrabuddhe C. G., Shaw B. R. Electron microscopy of chromatin subunit particles. Biochem Biophys Res Commun. 1974 Oct 23;60(4):1365–1370. doi: 10.1016/0006-291x(74)90348-9. [DOI] [PubMed] [Google Scholar]

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