Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1977 Apr;4(4):1001–1014. doi: 10.1093/nar/4.4.1001

Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.

R Contreras, G Volckaert, F Thys, A Van de Voorde, W Fiers
PMCID: PMC342500  PMID: 194225

Abstract

The nucleotide sequence of the SV40 genome region between the Hind K fragment and the Eco RI cleavage site has been determined by a combination of three different approaches : analysis of RNA products obtained by transcription with Escherichia coli DNA dependent RNA polymerase, partial degradations with snake venom exonuclease and base-specific chemical degradation of 5'-terminal labeled restriction fragments. This nucleotide sequence shows only one open reading frame and allows the deduction of a small segment of the amino acid sequence of VP1, the major structural protein.

Full text

PDF
1001

Images in this article

1004Image
on p.1004
1007Image
on p.1007
1009Image
on p.1009
1011Image
on p.1011

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Contreras R., Fiers W. A new method for partial digestion useful for sequence analysis of polynucleotides. FEBS Lett. 1971 Sep 1;16(4):281–283. doi: 10.1016/0014-5793(71)80370-8. [DOI] [PubMed] [Google Scholar]
  2. Danna K. J., Sack G. H., Jr, Nathans D. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol. 1973 Aug 5;78(2):363–376. doi: 10.1016/0022-2836(73)90122-8. [DOI] [PubMed] [Google Scholar]
  3. Dhar R., Zain S., Weissman S. M., Pan J., Subramanian K. Nucleotide sequences of RNA transcribed in infected cells and by Escherichia coli RNA polymerase from a segment of simian virus 40 DNA. Proc Natl Acad Sci U S A. 1974 Feb;71(2):371–375. doi: 10.1073/pnas.71.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Galibert F., Sedat J., Ziff E. Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA. J Mol Biol. 1974 Aug 15;87(3):377–407. doi: 10.1016/0022-2836(74)90093-x. [DOI] [PubMed] [Google Scholar]
  5. Garfin D. E., Boyer H. W., Goodman H. M. Sequences spanning the EcoRI substrate site. Nucleic Acids Res. 1975 Oct;2(10):1851–1865. doi: 10.1093/nar/2.10.1851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Girard M., Marty L., Suarez F. Capsid proteins of Simian virus 40. Biochem Biophys Res Commun. 1970 Jul 13;40(1):97–102. doi: 10.1016/0006-291x(70)91051-x. [DOI] [PubMed] [Google Scholar]
  7. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Greene P. H., Poonian M. S., Nussbaum A. L., Tobias L., Garfin D. E., Boyer H. W., Goodman H. M. Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate. J Mol Biol. 1975 Dec 5;99(2):237–261. doi: 10.1016/s0022-2836(75)80143-4. [DOI] [PubMed] [Google Scholar]
  9. Rozenblatt S., Mulligan R. C., Gorecki M., Roberts B. E., Rich A. Direct biochemical mapping of eukaryotic viral DNA by means of a linked transcription-translation cell-free system. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2747–2751. doi: 10.1073/pnas.73.8.2747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Simsek M., Ziegenmeyer J., Heckman J., Rajbhandary U. L. Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1041–1045. doi: 10.1073/pnas.70.4.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Van de Voorde A., Contreras R., Rogiers R., Fiers W. The initiation region of the SV40 VP1 gene. Cell. 1976 Sep;9(1):117–120. doi: 10.1016/0092-8674(76)90057-x. [DOI] [PubMed] [Google Scholar]
  12. Volckaert G., Jou W. M., Fiers W. Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure. Anal Biochem. 1976 May 7;72:433–446. doi: 10.1016/0003-2697(76)90551-0. [DOI] [PubMed] [Google Scholar]
  13. Yang R. C., Van de Voorde A., Fiers W. Cleavage map of the simian-virus-40 genome by the restriction endonuclease III of Haemopholus aegyptius. Eur J Biochem. 1976 Jan 2;61(1):101–117. doi: 10.1111/j.1432-1033.1976.tb10002.x. [DOI] [PubMed] [Google Scholar]
  14. Yang R. C., Van de Voorde A., Fiers W. Specific cleavage and physical mapping of simian-virus-40 DNA by the restriction endonuclease of Arthrobacter luteus. Eur J Biochem. 1976 Jan 2;61(1):119–138. doi: 10.1111/j.1432-1033.1976.tb10003.x. [DOI] [PubMed] [Google Scholar]
  15. de Wachter R., Merregaert J., Vandenberghe A., Contreras R., Fiers W. Studies on the bacteriophage MS2. The untranslated 5'-terminal nucleotide sequence preceding the first cistron. Eur J Biochem. 1971 Oct 14;22(3):400–414. doi: 10.1111/j.1432-1033.1971.tb01558.x. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES