Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1977 Jun;4(6):1783–1791. doi: 10.1093/nar/4.6.1783

A circular dichroism study of DNA-basic peptides associations in the absence or in the presence of Ca++.

C Lacombe, A Laigle
PMCID: PMC342521  PMID: 896474

Abstract

The interaction of DNA with basic peptides (Lys methyl ester*, Lys2, (Lys)2methyl ester) has been studied by circular dichroism. The changes of the DNA CD spectra in the presence of peptides are interpreted as a transconformation from the B form to the C form of DNA. The presence of Ca++ in the mixture induces a supplementary transconformation. These observations suggest Ca++-basic peptides-DNA complexes as a structural model for chromatin.

Full text

PDF
1783

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradbury E. M., Cary P. D., Crane-Robinson C., Rattle H. W., Boublik M., Sautière P. Conformations and interactions of histone H2A (F2A2, ALK). Biochemistry. 1975 May 6;14(9):1876–1885. doi: 10.1021/bi00680a012. [DOI] [PubMed] [Google Scholar]
  2. Bram S. The function of the structure of DNA in chromosomes. Biochimie. 1972;54(8):1005–1011. doi: 10.1016/s0300-9084(72)80051-8. [DOI] [PubMed] [Google Scholar]
  3. Bram S. The secondary structure of DNA in solution and in nucleohistone. J Mol Biol. 1971 May 28;58(1):277–288. doi: 10.1016/0022-2836(71)90246-4. [DOI] [PubMed] [Google Scholar]
  4. Bram S., Tougard P. Polymorphism of natural DNA. Nat New Biol. 1972 Oct 4;239(92):128–131. doi: 10.1038/newbio239128a0. [DOI] [PubMed] [Google Scholar]
  5. Chang C., Weiskopf M., Li H. J. Conformational studies of nucleoprotein. Circular dichroism of deoxyribonucleic acid base pairs bound by polylysine. Biochemistry. 1973 Jul 31;12(16):3028–3032. doi: 10.1021/bi00740a013. [DOI] [PubMed] [Google Scholar]
  6. Chang C., Weiskopf M., Li H. J. Conformational studies of nucleoprotein. Circular dichroism of deoxyribonucleic acid base pairs bound by polylysine. Biochemistry. 1973 Jul 31;12(16):3028–3032. doi: 10.1021/bi00740a013. [DOI] [PubMed] [Google Scholar]
  7. Cheng S. M., Mohr S. C. The thermal transition of 'psi' DNA monitored by circular dichroism. FEBS Lett. 1974 Dec 1;49(1):37–42. doi: 10.1016/0014-5793(74)80626-5. [DOI] [PubMed] [Google Scholar]
  8. Gourévitch M., Puigdoménech P., Cavé A., Etienne G., Méry J., Parello J. Model studies in relation to the molecular structure of chromatin. Biochimie. 1974;56(6-7):967–985. doi: 10.1016/s0300-9084(74)80518-3. [DOI] [PubMed] [Google Scholar]
  9. Hanlon S., Brudno S., Wu T. T., Wolf B. Structural transitions of deoxyribonucleic acid in aqueous electrolyte solutions. I. Reference spectra of conformational limits. Biochemistry. 1975 Apr 22;14(8):1648–1660. doi: 10.1021/bi00679a017. [DOI] [PubMed] [Google Scholar]
  10. Hanlon S., Johnson R. S., Chan A. Relationship between protein and DNA structure in calf thymus chromatin. II. Conformational aspects. Biochemistry. 1974 Sep 10;13(19):3972–3981. doi: 10.1021/bi00716a024. [DOI] [PubMed] [Google Scholar]
  11. Hjelm R. P., Jr, Huang R. C. The contribution of RNA and non-histone proteins to the circular dichroism spectrum of chromatin. Biochemistry. 1975 Apr 22;14(8):1682–1688. doi: 10.1021/bi00679a021. [DOI] [PubMed] [Google Scholar]
  12. Hjelm R. P., Jr, Huang R. C. The role of histones in the conformation of DNA in chromatin as studied by circular dichroism. Biochemistry. 1974 Dec 17;13(26):5275–5283. doi: 10.1021/bi00723a004. [DOI] [PubMed] [Google Scholar]
  13. Lacombe C., Cattan D., Laigle A. Associations DNA-Ribonucléase. 1. Etude physico-chimique. Biochimie. 1974;56(3):341–346. doi: 10.1016/s0300-9084(74)80141-0. [DOI] [PubMed] [Google Scholar]
  14. Lacombe C., Cattan D., Laigle A. Associations DNA-ribonucléase. II - Etude conformationnelle. Biochimie. 1974;56(5):649–657. doi: 10.1016/s0300-9084(74)80035-0. [DOI] [PubMed] [Google Scholar]
  15. Li H. J., Chang C., Evagelinou Z., Weiskopf M. Circular dichroism of histone-bound regions in chromatin. Biopolymers. 1975 Jan;14(1):211–226. doi: 10.1002/bip.1975.360140115. [DOI] [PubMed] [Google Scholar]
  16. Liquier J., Pinot-Lafaix M., Taillandier E., Brahms J. Infrared linear dichroism investigations of deoxyribonucleic acid complexes with poly(L-arginine) and poly(L-lysine). Biochemistry. 1975 Sep 23;14(19):4191–4197. doi: 10.1021/bi00690a007. [DOI] [PubMed] [Google Scholar]
  17. Olins D. E., Olins A. L., Von Hippel P. H. Model nucleoprotein complexes: studies on the interaction of cationic homopolypeptides with DNA. J Mol Biol. 1967 Mar 14;24(2):157–176. doi: 10.1016/0022-2836(67)90324-5. [DOI] [PubMed] [Google Scholar]
  18. Shih T. Y., Fasman G. D. Conformation of deoxyribonucleic acid in chromatin: a circular dichroism study. J Mol Biol. 1970 Aug 28;52(1):125–129. doi: 10.1016/0022-2836(70)90182-8. [DOI] [PubMed] [Google Scholar]
  19. Simpson R. T., Sober H. A. Circular dichroism of calf liver nucleohistone. Biochemistry. 1970 Aug 4;9(16):3103–3109. doi: 10.1021/bi00818a001. [DOI] [PubMed] [Google Scholar]
  20. Tashiro T., Kurokawa M. A contribution of nonhistone proteins to the conformation of chromatin. Eur J Biochem. 1975 Dec 15;60(2):569–577. doi: 10.1111/j.1432-1033.1975.tb21035.x. [DOI] [PubMed] [Google Scholar]
  21. Wagner T. E., Vandegrift V. Circular dichrosim studies of calf thymus Ca 2+ nucleohistone IV. Biochemistry. 1972 Apr 11;11(8):1431–1436. doi: 10.1021/bi00758a016. [DOI] [PubMed] [Google Scholar]
  22. Weintraub H. Release of discrete subunits after nuclease and trypsin digestion of chromatin. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1212–1216. doi: 10.1073/pnas.72.3.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yu S. S., Epstein P., Li H. J. Helix-coil transition and conformational studies of nucleoprotein: poly(L-arginine)- and poly(L-arginine87, L-ornithine13)-DNA complexes. II. Circular dichroism. Biochemistry. 1974 Aug 27;13(18):3713–3717. doi: 10.1021/bi00715a015. [DOI] [PubMed] [Google Scholar]
  24. Zama M. Structure and circular dichroism of DNA--polylysine--polyarginine complex. Biochim Biophys Acta. 1974 Oct 11;366(2):124–134. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES