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. 1977 Jun;4(6):1837–1851. doi: 10.1093/nar/4.6.1837

Salt and divalent cations affect the flexible nature of the natural beaded chromatin structure.

G Christiansen, J Griffith
PMCID: PMC342526  PMID: 197496

Abstract

A natural chromatin containing simian virus 40 (SV40) DNA and histone has been used to examine changes in chromatin structure caused by various physical and chemical treatments. We find that histone H1 depleted chromatin is more compact in solutions of 0.15M NaCl or 2 mM MgCl2 than in 0.01 M NaCl or 0.6M NaCL, and is compact in 0.01 M NaCl solutions if histone H 1 is present. Even high concentrations of urea did not alter the fundamental beaded structure, consisting of 110A beads of 200 base pair content, each joined by thin DNA bridges of 50 base pairs. The physical bead observed by EM therefore contains more DNA than the 140 base pair "core particle". The natural variation in the bridge length is consistent with the broad bands observed after nuclease digestion of chromatin. Chromatin prepared for EM without fixation containing long 20A to 30A fibers possibly complexed with protein.

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Selected References

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  1. Bartley J., Chalkley R. An approach to the structure of native nucleohistone. Biochemistry. 1973 Jan 30;12(3):468–474. doi: 10.1021/bi00727a017. [DOI] [PubMed] [Google Scholar]
  2. Bellard M., Oudet P., Germond J. E., Chambon P. Subunit structure of simian-virus-40 minichromosome. Eur J Biochem. 1976 Nov 15;70(2):543–553. doi: 10.1111/j.1432-1033.1976.tb11046.x. [DOI] [PubMed] [Google Scholar]
  3. Blobel G., Potter V. R. Nuclei from rat liver: isolation method that combines purity with high yield. Science. 1966 Dec 30;154(3757):1662–1665. doi: 10.1126/science.154.3757.1662. [DOI] [PubMed] [Google Scholar]
  4. Brutlag D., Schlehuber C., Bonner J. Properties of formaldehyde-treated nucleohistone. Biochemistry. 1969 Aug;8(8):3214–3218. doi: 10.1021/bi00836a013. [DOI] [PubMed] [Google Scholar]
  5. Chalkley R., Hunter C. Histone-histone propinquity by aldehyde fixation of chromatin. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1304–1308. doi: 10.1073/pnas.72.4.1304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cremisi C., Pignatti P. F., Croissant O., Yaniv M. Chromatin-like structures in polyoma virus and simian virus 10 lytic cycle. J Virol. 1975 Jan;17(1):204–211. doi: 10.1128/jvi.17.1.204-211.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Germond J. E., Hirt B., Oudet P., Gross-Bellark M., Chambon P. Folding of the DNA double helix in chromatin-like structures from simian virus 40. Proc Natl Acad Sci U S A. 1975 May;72(5):1843–1847. doi: 10.1073/pnas.72.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Griffith J. D. Chromatin structure: deduced from a minichromosome. Science. 1975 Mar 28;187(4182):1202–1203. doi: 10.1126/science.187.4182.1202. [DOI] [PubMed] [Google Scholar]
  9. Griffith J. D. Electron microscopic visualization of DNA in association with cellular components. Methods Cell Biol. 1973;7:129–146. doi: 10.1016/s0091-679x(08)61774-4. [DOI] [PubMed] [Google Scholar]
  10. Griffith J., Dieckmann M., Berg P. Electron microscope localization of a protein bound near the origin of simian virus 40 DNA replication. J Virol. 1975 Jan;15(1):167–172. doi: 10.1128/jvi.15.1.167-172.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kornberg R. D. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. doi: 10.1126/science.184.4139.868. [DOI] [PubMed] [Google Scholar]
  12. Lake R. S., Barban S., Salzman N. P. Resolutions and identification of the core deoxynucleoproteins of the simian virus 40. Biochem Biophys Res Commun. 1973 Sep 18;54(2):640–647. doi: 10.1016/0006-291x(73)91471-x. [DOI] [PubMed] [Google Scholar]
  13. Meinke W., Hall M. R., Goldstein D. A. Proteins in intracellular simian virus 40 nucleoportein complexes: comparison with simian virus 40 core proteins. J Virol. 1975 Mar;15(3):439–448. doi: 10.1128/jvi.15.3.439-448.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Noll M., Thomas J. O., Kornberg R. D. Preparation of native chromatin and damage caused by shearing. Science. 1975 Mar 28;187(4182):1203–1206. doi: 10.1126/science.187.4182.1203. [DOI] [PubMed] [Google Scholar]
  15. Pett D. M., Estes M. K., Pagano J. S. Structural proteins of simian virus 40. I. Histone characteristics of low-molecular-weight polypeptides. J Virol. 1975 Feb;15(2):379–385. doi: 10.1128/jvi.15.2.379-385.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shaw B. R., Corden J. L., Sahasrabuddhe C. G., Van Holde K. E. Chromatographic separation of chromatin subunits. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1193–1198. doi: 10.1016/s0006-291x(74)80410-9. [DOI] [PubMed] [Google Scholar]
  17. Sollner-Webb B., Felsenfeld G. A comparison of the digestion of nuclei and chromatin by staphylococcal nuclease. Biochemistry. 1975 Jul;14(13):2915–2920. doi: 10.1021/bi00684a019. [DOI] [PubMed] [Google Scholar]
  18. Varshavsky A. J., Bakayev V. V., Chumackov P. M., Georgiev G. P. Minichromosome of simian virus 40: presence of histone HI. Nucleic Acids Res. 1976 Aug;3(8):2101–2113. doi: 10.1093/nar/3.8.2101. [DOI] [PMC free article] [PubMed] [Google Scholar]

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