Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1977 Jun;4(6):1873–1880. doi: 10.1093/nar/4.6.1873

In vivo repair of the 3'terminus of transfer RNA injected into amphibian oocytes.

A Solari, M Gatica, J E Allende
PMCID: PMC342528  PMID: 896475

Abstract

Yeast transfer RNA specific for phenylalanine has been treated chemically to remove either one or two nucleotides of its 3' terminus and has been injected into Xenopus laevis oocytes to test whether this RNA can be repaired in vivo. The results obtained showed that oocytes could aminoacylate and thus repair tRNAPhe that has lost both its terminal adenosine and 3' phosphate. A similar result was obtained with tRNAPhe that had undergone two full cycles of 3' terminal nucleotide removal. The oocytes cannot aminoacylate tRNAPhe whose 3' terminal ribose has been oxidized with periodate or the derivative that retains a 3' phosphate after adenosine removal. In vitro assays show that the Xenopus ovary contains a tRNA nucleotidyl transferase with the properties similar to enzymes obtained from other sources which may be responsible for the 3' terminal repair observed in vitro.

Full text

PDF
1873

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allende C. C., Allende J. E., Firtel R. A. The degradation of ribonucleic acids injected into Xenopus laevis oocytes. Cell. 1974 Jul;2(3):189–196. doi: 10.1016/0092-8674(74)90093-2. [DOI] [PubMed] [Google Scholar]
  2. DANIEL V., LITTAUER U. Z. ENZYMIC INCORPORATION OF RIBONUCLEOTIDES AND AMINO ACIDS INTO TRANSFER RIBONUCLEIC ACID. J Mol Biol. 1965 Apr;11:692–705. doi: 10.1016/s0022-2836(65)80027-4. [DOI] [PubMed] [Google Scholar]
  3. Deutscher M. P., Foulds J., McClain W. H. Transfer ribonucleic acid nucleotidyl-transferase plays an essential role in the normal growth of Escherichia coli and in the biosynthesis of some bacteriophage T4 transfer ribonucleic acids. J Biol Chem. 1974 Oct 25;249(20):6696–6699. [PubMed] [Google Scholar]
  4. Deutscher M. P. Reactions at the 3' terminus of transfer ribonucleic acid. 3. Catalytic properties of two purified rabbit liver transfer ribonucleic acid nucleotidyl transferases. J Biol Chem. 1972 Jan 25;247(2):459–468. [PubMed] [Google Scholar]
  5. Deutscher M. P. Synthesis and functions of the -C-C-A terminus of transfer RNA. Prog Nucleic Acid Res Mol Biol. 1973;13:51–92. doi: 10.1016/s0079-6603(08)60100-2. [DOI] [PubMed] [Google Scholar]
  6. Gatica M., Tarragó A., Allende C. C., Allende J. E. Aminoacylation of transfer RNA microinjected into Xenopus laevis oocytes. Nature. 1975 Aug 21;256(5519):675–678. doi: 10.1038/256675a0. [DOI] [PubMed] [Google Scholar]
  7. Gurdon J. B. Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes. J Embryol Exp Morphol. 1968 Nov;20(3):401–414. [PubMed] [Google Scholar]
  8. Lane C. D., Marbaix G., Gurdon J. B. Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes. J Mol Biol. 1971 Oct 14;61(1):73–91. doi: 10.1016/0022-2836(71)90207-5. [DOI] [PubMed] [Google Scholar]
  9. Laskey R. A., Gurdon J. B., Crawford L. V. Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3665–3669. doi: 10.1073/pnas.69.12.3665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Paradiso P., Schofield P. Changes in tRNA nucleotidyl transferase activity during embryonic development of Xenopus laevis. Exp Cell Res. 1976 Jun;100(1):9–14. doi: 10.1016/0014-4827(76)90320-7. [DOI] [PubMed] [Google Scholar]
  11. Woodland H. R., Pestell R. Q. Determination of the nucleoside triphosphate contents of eggs and oocytes of Xenopus laevis. Biochem J. 1972 Apr;127(3):597–605. doi: 10.1042/bj1270597. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES