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. 1977 Jun;4(6):2029–2037. doi: 10.1093/nar/4.6.2029

Levels of DNA polymerases alpha, beta, and gamma in control and repair-deficient human diploid fibroblasts 1.

V P Parker, M W Lieberman
PMCID: PMC342540  PMID: 896483

Abstract

The activities of DNA polymerases alpha, beta, and gamma were determined in control and repair-deficient human fibroblasts (xeroderma pigmentosum complementation groups A, C, and D; Fanconi's Anemia; and Bloom's syndrome). Assays were done on 103,000XG supernatants which had been chromatographed on DEAE cellulose to remove nucleic acids and on fractions containing polymerase activities which had been separated from one another on a second DEAE cellulose column. All repair-deficient cell types contained all three DNA polymerase activities. Caffeine, which has been observed to inhibit some DNA-repair processes in intact cells, had no effect on DNA polymerase activities from XP-A, XP-C, XP-D or XP-variant cells. These data indicate that all three polymerases are present in cells which have reduced or absent repair functions and that the caffeine effects observed in living cells are probably not due to the direct action of caffeine on DNA polymerases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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