Abstract
Alpha-lactalbumin messenger RNA was partially purified from RNA extracted from 3-5 day lactating rat mammary glands on a poly(U)-sepharose column followed by sucrose gradient centrifugation. Apha-Lactalbumin mRNA activity was assayed in wheat germ cell-free translational system by immunoprecipitation of the in vitro synthesized protein using specific antiserum prepared against purified rat alpha-lactalbumin. In the purified mRNA preparation alpha-lactalbumin mRNA activity comprised approximately 85% of the total mRNA activity.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brodbeck U., Ebner K. E. Resolution of a soluble lactose synthetase into two protein components and solubilization of microsomal lactose synthetase. J Biol Chem. 1966 Feb 10;241(3):762–764. [PubMed] [Google Scholar]
- Chan S. J., Keim P., Steiner D. F. Cell-free synthesis of rat preproinsulins: characterization and partial amino acid sequence determination. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1964–1968. doi: 10.1073/pnas.73.6.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dingman C. W., Peacock A. C. Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis. Biochemistry. 1968 Feb;7(2):659–668. doi: 10.1021/bi00842a022. [DOI] [PubMed] [Google Scholar]
- Kern H. Fractionation of nucleic acids with special regard to rapidly labeled RNA. Anal Biochem. 1975 Jul;67(1):147–156. doi: 10.1016/0003-2697(75)90282-1. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Landefeld T. D., McWilliams D. R., Boime I. The isolation of mRNA encoding the alpha subunit of human chorionic gonadotropin. Biochem Biophys Res Commun. 1976 Sep 20;72(2):381–390. doi: 10.1016/s0006-291x(76)80054-x. [DOI] [PubMed] [Google Scholar]
- Lebleu B. Isolation of rabbit reticulocyte 9 S mRNA. Methods Enzymol. 1974;30:613–621. doi: 10.1016/0076-6879(74)30059-6. [DOI] [PubMed] [Google Scholar]
- Lindberg U., Persson T., Philipson L. Isolation and characterization of adenovirus messenger ribonucleic acid in productive infection. J Virol. 1972 Nov;10(5):909–919. doi: 10.1128/jvi.10.5.909-919.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosen J. M., Woo S. L., Comstock J. P. Regulation of casein messenger RNA during the development of the rat mammary gland. Biochemistry. 1975 Jul;14(13):2895–2903. doi: 10.1021/bi00684a016. [DOI] [PubMed] [Google Scholar]