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. 1977 Jun;4(6):2075–2081. doi: 10.1093/nar/4.6.2075

Incorporation of oxygen-18 into nucleosides and bases.

G Puzo, K H Schram, J A McCloskey
PMCID: PMC342544  PMID: 896486

Abstract

Extensive incorporation of oxygen-18 at position O4 of the pyrimidine nucleus results from exchange between H218O and nucleosides or bases in 1N HC1 at 100 degrees. The reaction is hindered by substitution at C-5 with the greatest effect shown in pseudouridine (R = ribosy1) and the least in uridine (R = H). Maximum incorporation in the latter compound was 94%, and in uricil was 98%. The method is experimentally simple and the incorporation is readily monitored by mass spectrometry.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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