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. 1977 Jul;4(7):2275–2282. doi: 10.1093/nar/4.7.2275

Chromatin very small angle neutron scattering: further evidence for a 30 nm diameter super coil in dilute solutions.

S Bram, P Baudy, J Lepault, D Hermann
PMCID: PMC342565  PMID: 909775

Abstract

Intact chromatin, chromatin minus histone H1, and nuclease digestion fragments have been studied by very small angle neutron scattering. The results are not consistent with a straight chain of nucleosomes and require the presence of a higher order coiling in monovalent salt solutions. The data are interpretable by a structure having a cross section radius of gryration of 8.5 +/- 1 nm, which suggests an outer diameter for a coil of nucleosomes of 27 +/- 3 nm.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baudy P., Bram S., Vastel D., Lepault J. Chromatin subunit small angle neutron scattering: a DNA rich coil surrounds a protein-DNA core. Biochem Biophys Res Commun. 1976 Sep 7;72(1):176–183. doi: 10.1016/0006-291x(76)90976-1. [DOI] [PubMed] [Google Scholar]
  2. Bram S. A double coil chromatin sub-unit model. Biochimie. 1975 Nov-Dec;57(11-12):1301–1306. doi: 10.1016/s0300-9084(76)80542-1. [DOI] [PubMed] [Google Scholar]
  3. Bram S., Butler-Browne G., Baudy P., Ibel K. Quaternary structure of chromatin. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1043–1045. doi: 10.1073/pnas.72.3.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bram S., Ris H. On the structure of nucleohistone. J Mol Biol. 1971 Feb 14;55(3):325–336. doi: 10.1016/0022-2836(71)90321-4. [DOI] [PubMed] [Google Scholar]
  5. Carlson R. D., Olins D. E. Chromatin model calculations: Arrays of spherical nu bodies. Nucleic Acids Res. 1976 Jan;3(1):89–100. doi: 10.1093/nar/3.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carpenter B. G., Baldwin J. P., Bradbury E. M., Ibel K. Organisation of subunits in chromatin. Nucleic Acids Res. 1976 Jul;3(7):1739–1746. doi: 10.1093/nar/3.7.1739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Finch J. T., Klug A. Solenoidal model for superstructure in chromatin. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1897–1901. doi: 10.1073/pnas.73.6.1897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hewish D. R., Burgoyne L. A. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochem Biophys Res Commun. 1973 May 15;52(2):504–510. doi: 10.1016/0006-291x(73)90740-7. [DOI] [PubMed] [Google Scholar]
  9. Kornberg R. D., Thomas J. O. Chromatin structure; oligomers of the histones. Science. 1974 May 24;184(4139):865–868. doi: 10.1126/science.184.4139.865. [DOI] [PubMed] [Google Scholar]
  10. Noll M., Thomas J. O., Kornberg R. D. Preparation of native chromatin and damage caused by shearing. Science. 1975 Mar 28;187(4182):1203–1206. doi: 10.1126/science.187.4182.1203. [DOI] [PubMed] [Google Scholar]
  11. Oudet P., Gross-Bellard M., Chambon P. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975 Apr;4(4):281–300. doi: 10.1016/0092-8674(75)90149-x. [DOI] [PubMed] [Google Scholar]
  12. Panyim S., Bilek D., Chalkley R. An electrophoretic comparison of vertebrate histones. J Biol Chem. 1971 Jul 10;246(13):4206–4215. [PubMed] [Google Scholar]
  13. Rill R., Van Holde K. E. Properties of nuclease-resistant fragments of calf thymus chromatin. J Biol Chem. 1973 Feb 10;248(3):1080–1083. [PubMed] [Google Scholar]
  14. Shaw B. R., Schmitz K. S. Quasielastic light scattering by biopolymers. Conformation of chromatin multimers. Biochem Biophys Res Commun. 1976 Nov 22;73(2):224–232. doi: 10.1016/0006-291x(76)90697-5. [DOI] [PubMed] [Google Scholar]
  15. Sperling L. The mass per unit length of chromatin by low-angle x-ray scattering. FEBS Lett. 1976 Apr 15;64(1):89–91. doi: 10.1016/0014-5793(76)80256-6. [DOI] [PubMed] [Google Scholar]

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