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. 1977 Jul;4(7):2369–2388. doi: 10.1093/nar/4.7.2369

The repeating nucleotide sequence in the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast.

C F Van Kreijl, J L Bos
PMCID: PMC342572  PMID: 198740

Abstract

The repeating nucleotide sequence of 68 base pairs in the mtDNA from an ethidium-induced cytoplasmic petite mutant of yeast has been determined. For sequence analysis specifically primed and terminated RNA copies, obtained by in vitro transcription of the separated strands, were use. The sequence consists of 66 consecutive AT base pairs flanked by two GC pairs and comprises nearly all of the mutant mitochondrial genome. The sequence, moreover, also represents the first part of wild-type mtDNA sequence so far.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borst P. Mitochondrial nucleic acids. Annu Rev Biochem. 1972;41:333–376. doi: 10.1146/annurev.bi.41.070172.002001. [DOI] [PubMed] [Google Scholar]
  2. Brownlee G. G., Sanger F. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem. 1969 Dec;11(2):395–399. doi: 10.1111/j.1432-1033.1969.tb00786.x. [DOI] [PubMed] [Google Scholar]
  3. Hall R. M., Nagley P., Linnane A. W. Biogenesis of mitochondria. XLII. Genetic analysis of the control of cellular mitochondrial DNA levels in Saccharomyces cerevisiae. Mol Gen Genet. 1976 May 7;145(2):169–175. doi: 10.1007/BF00269590. [DOI] [PubMed] [Google Scholar]
  4. Hollenberg C. P., Borst P., Flavell R. A., van Kreijl C. F., van Bruggen E. F., Arnberg A. C. The unusual properties of mtDNA from a "low-density" petite mutant of yeast. Biochim Biophys Acta. 1972 Aug 16;277(1):44–58. doi: 10.1016/0005-2787(72)90350-4. [DOI] [PubMed] [Google Scholar]
  5. Mol J. N., Borst P., Grosveld F. G., Spencer J. H. The size of the repeating unit of the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast. Biochim Biophys Acta. 1974 Dec 6;374(2):115–128. doi: 10.1016/0005-2787(74)90355-4. [DOI] [PubMed] [Google Scholar]
  6. Polisky B., Greene P., Garfin D. E., McCarthy B. J., Goodman H. M., Boyer H. W. Specificity of substrate recognition by the EcoRI restriction endonuclease. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310–3314. doi: 10.1073/pnas.72.9.3310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Prunell A., Bernardi G. The mitochondrial genome of wild-type yeast cells. IV. Genes and spacers. J Mol Biol. 1974 Jul 15;86(4):825–841. doi: 10.1016/0022-2836(74)90356-8. [DOI] [PubMed] [Google Scholar]
  8. Rogg H., Staehelin M. On the specificity of ribonuclease U 2 . Biochim Biophys Acta. 1972 Mar 24;262(3):314–319. doi: 10.1016/0005-2787(72)90268-7. [DOI] [PubMed] [Google Scholar]
  9. Sanders J. P., Flavell R. A., Borst P., Mol J. N. Nature of the base sequence conserved in the mitochondrial DNA of a low-density petite. Biochim Biophys Acta. 1973 Jul 13;312(3):441–457. doi: 10.1016/0005-2787(73)90443-7. [DOI] [PubMed] [Google Scholar]
  10. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  11. Van Kreijl C. F., Beelen R. H., Borst P. On the mechanism of oligonucleotide-primed RNA synthesis. I. Model studies with deoxyhomopolymer templates and Escherichia coli RNA polymerase. Nucleic Acids Res. 1977 Feb;4(2):425–444. doi: 10.1093/nar/4.2.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Van Kreijl C. F., Beelen R. H., Borst P. On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis. Nucleic Acids Res. 1977 Feb;4(2):445–455. doi: 10.1093/nar/4.2.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Volckaert G., Jou W. M., Fiers W. Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure. Anal Biochem. 1976 May 7;72:433–446. doi: 10.1016/0003-2697(76)90551-0. [DOI] [PubMed] [Google Scholar]
  14. de Wachter R., Fiers W. Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA. Anal Biochem. 1972 Sep;49(1):184–197. doi: 10.1016/0003-2697(72)90257-6. [DOI] [PubMed] [Google Scholar]
  15. van Kreijl C. F., Borst P., Flavell R. A., Hollenberg C. P. Pyrimidine tract analysis of mtDNA from a "low-density" petite mutant of yeast. Biochim Biophys Acta. 1972 Aug 16;277(1):61–70. doi: 10.1016/0005-2787(72)90352-8. [DOI] [PubMed] [Google Scholar]

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