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. 1977 Jul;4(7):2495–2505. doi: 10.1093/nar/4.7.2495

Nucleotide sequence relationships between vertebrate 5.8 S ribosomal RNAs.

M S Khan, B E Maden
PMCID: PMC342582  PMID: 409998

Abstract

Nucleotide sequences of the 5.8 S ribosomal RNAs from HeLa cells, Xenopus laevis and chick embryo fibroblasts were compared. Xenopus laevis 5.8 S RNA differs from that of HeLa cells in four internal positions and at the 3' end of the molecule. Chick 5.8 S RNA differs from that of HeLa cells in two positions. Six out of the seven interspecies differences are due to base substitutions. The other difference is due to the presence of an extra nucleotide, internally located, within the Xenopus 5.8 S sequence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hampe A., Eladari M. E., Galibert F. Nucleotide sequence study of mouse 5.8S ribosomal RNA. Biochimie. 1976;58(8):943–951. doi: 10.1016/s0300-9084(76)80282-9. [DOI] [PubMed] [Google Scholar]
  2. Khan M. S., Maden B. E. Conformation of mammalian 5.8 S ribosomal RNA: S1 nuclease as a probe. FEBS Lett. 1976 Dec 15;72(1):105–110. doi: 10.1016/0014-5793(76)80823-x. [DOI] [PubMed] [Google Scholar]
  3. Khan M. S., Maden B. E. Nucleotide sequences within the ribosomal ribonucleic acids of HeLa cells, Xenopus laevis and chick embryo fibroblasts. J Mol Biol. 1976 Feb 25;101(2):235–254. doi: 10.1016/0022-2836(76)90375-2. [DOI] [PubMed] [Google Scholar]
  4. King H. W., Gould H. Low molecular weight ribonucleic acid in rabbit reticulocyte ribosomes. J Mol Biol. 1970 Aug;51(3):687–702. doi: 10.1016/0022-2836(70)90017-3. [DOI] [PubMed] [Google Scholar]
  5. Maden B. E., Robertson J. S. Demonstration of the "5-8 S" ribosomal sequence in HeLa cell ribosomal precursor RNA. J Mol Biol. 1974 Aug 5;87(2):227–235. doi: 10.1016/0022-2836(74)90145-4. [DOI] [PubMed] [Google Scholar]
  6. Nazar R. N., Owens T. W., Sitz T. O., Busch H. Maturation pathway for Novikoff ascites hepatoma 5.8 S ribosomal ribonucleic acid. Evidence for its presence in 32 S nuclear ribonucleic acid. J Biol Chem. 1975 Apr 10;250(7):2475–2481. [PubMed] [Google Scholar]
  7. Nazar R. N., Roy K. L. The nucleotide sequence of turtle 5.8 s rRNA. FEBS Lett. 1976 Dec 15;72(1):111–116. doi: 10.1016/0014-5793(76)80824-1. [DOI] [PubMed] [Google Scholar]
  8. Nazar R. N., Sitz T. O., Busch H. Sequence homologies in mammalian 5.8S ribosomal RNA. Biochemistry. 1976 Feb 10;15(3):505–508. doi: 10.1021/bi00648a008. [DOI] [PubMed] [Google Scholar]
  9. Nazar R. N., Sitz T. O., Busch H. Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid. J Biol Chem. 1975 Nov 25;250(22):8591–8597. [PubMed] [Google Scholar]
  10. Nazar R. N., Sitz T. O., Busch H. Tissue specific differences in the 2'-O-methylation of eukaryotic 5.8S ribosomal RNA. FEBS Lett. 1975 Nov 1;59(1):83–87. doi: 10.1016/0014-5793(75)80346-2. [DOI] [PubMed] [Google Scholar]
  11. Pene J. J., Knight E., Jr, Darnell J. E., Jr Characterization of a new low molecular weight RNA in HeLa cell ribosomes. J Mol Biol. 1968 May 14;33(3):609–623. doi: 10.1016/0022-2836(68)90309-4. [DOI] [PubMed] [Google Scholar]
  12. Rubin G. M. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem. 1973 Jun 10;248(11):3860–3875. [PubMed] [Google Scholar]
  13. Speirs J., Birnstiel M. Arrangement of the 5-8 S RNA cistrons in the genome of Xenopus laevis. J Mol Biol. 1974 Aug 5;87(2):237–256. doi: 10.1016/0022-2836(74)90146-6. [DOI] [PubMed] [Google Scholar]
  14. Tinoco I., Jr, Uhlenbeck O. C., Levine M. D. Estimation of secondary structure in ribonucleic acids. Nature. 1971 Apr 9;230(5293):362–367. doi: 10.1038/230362a0. [DOI] [PubMed] [Google Scholar]
  15. Walker T. A., Pace N. R. Transcriptional organization of the 5.8S ribosomal RNA cistron in Xenopus laevis ribosomal DNA. Nucleic Acids Res. 1977 Mar;4(3):595–601. doi: 10.1093/nar/4.3.595. [DOI] [PMC free article] [PubMed] [Google Scholar]

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