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. 1977 Aug;4(8):2641–2653. doi: 10.1093/nar/4.8.2641

Action of pancreatic DNase: requirements for activation of DNA as a template-primer for DNA polymerase.

E Baril, J Mitchener, L Lee, B Baril
PMCID: PMC342598  PMID: 410000

Abstract

Pancreatic DNase requires both Ca2+ and Mg2+ for its activity as measured by formation of an activated DNA template for in vitro DNA polymerase alpha assay and by the hyperchromic shift. Mn2+ can partially satisfy the Mg2+ requirement of the DNase for activation of DNA but the resulting template is only 50% as active in the DNA polymerase assay. When precautions are taken to avoid divalent ion contamination, pancreatic DNase is not active in the presence of Ca2+ or Mg2+ alone. analysis of the DNA by sucrose gradient centrifugation shows that only in the presence of Ca2+ plus Mg2+ or Mn2+ does pancreatic DNase produce extensive strand breaks in the DNA. The activated DNA template that yields maximal DNA polymerase activity is low molecular weight material of 30,000 to 50,000 daltons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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