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. 1977 Aug;4(8):2671–2682. doi: 10.1093/nar/4.8.2671

Methylated constituents of Aedes albopictus poly (A)-containing messenger RNA.

C C HsuChen, D T Dubin
PMCID: PMC342600  PMID: 198748

Abstract

Poly (A)-containing mRNA prepared from cultured mosquito (Aedes albopictus) cells was found to contain methylated 5'-terminal "caps" as well as internal m6A residues. Both type I [m7G(5')ppp(5')Xmp] and type II [m7G(5')ppp(5')XmpYmp] caps were present, at molar ratio of ca five to one. All four common RNA bases were represented in the second position (Xm) of the caps, adenine being the most abundant and N6-methyladenine being absent. The four bases were also represented in the third position (Ym), but here uracil was the predominant base. There was approximately one internal m6A residue for every three caps. These studies demonstrate that mRNA from an invertebrate source can have a methylation pattern comparable with that of mammalian cells in it complexity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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