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. 1977 Aug;4(8):2713–2723. doi: 10.1093/nar/4.8.2713

Early effects of phytohemagglutinin on induction of DNA polymerase, thymidine kinase, deoxyribonucleoside triphosphate pools and DNA synthesis in human lymphocytes.

G Tyrsted, B Munch-Petersen
PMCID: PMC342603  PMID: 909790

Abstract

In phytohemagglutinin stimulated human lymphocytes the time relationship was determined between induction of the parameters mentioned. The results indicate that the induction occurred in a specific sequence. Thus, a simultaneous increase in the activity of DNA polymerase and thymidinekinase occurred after 15 h of incubation with Phytohemagglutinin. Furthermore, this enhancement occurred 2 h before the expansion of the TTP and dCTP pools and 4 h before the expansion of the dATP and dGTP pools. The rate of [3H] deoxyguanosine incorporation into DNA increased simultaneously with the expansion of the TTP and dCTP pools.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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