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. 1977 Aug;4(8):2767–2777. doi: 10.1093/nar/4.8.2767

Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-s.

J T Sági, A Szabolcs, A Szemzö, L Otvös
PMCID: PMC342607  PMID: 333394

Abstract

The chemical synthesis of 5-alkyl-dUTP-s and their participation as substrates in poly[d(A-6)] primed polymerization reactions with dATP by E. coli DNA polymerase I enzyme has been described. In comparison with dTTP, at saturating substrate concentrations, the rate of hypochromic effect was found to be 17.3% higher for dUTP and was lower by 27.4% for 5-ethyl-dUTP, 29.5% for 5-n-propyl-dUTP, 31.4% for 5-n-butyl-dUTP and by 85.0% for 5-n-pentyl-dUTP. No hypochromic effect could be observed, however, with 5-iso-propyl-, 5-tert.butyl- and 5-n-hexyl-dUTP-s. Polydeoxynucleotides have also been isolated from the reaction mixture and some of their structural properties determined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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