Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1977 Aug;4(8):2799–2809. doi: 10.1093/nar/4.8.2799

Search for revertants of the glutamine mischarging mutans of Escherichia coli su+3 tyrosine suppressor tRNA that are able to insert tyrosine at the site of amber mutation.

J E Celis, M Squire, K Kaltoft, E Riisom
PMCID: PMC342609  PMID: 410001

Abstract

We have isolated a bacterial amber mutation (nadam) that is suppressed by the tyrosine inserting suppressor su+3 but not by the glutamine (su+2, su+3 A1, su+3 G82 and su+3 A1G82), serine (su+1) and leucine (su+6) inserting suppressors. The su+7 suppressor which inserts glutamine and tryptophan also suppresses this mutation indicating that tryptophan, in addition to tyrosine, is accepted at the site of amber mutation. We have used this amber mutation to search for revertants of the su+3 glutamine mischarging mutants su+3 A1, su+3 G82 and su+3 A1G82 that are able to insert tyrosine at the site of amber mutation. Two types of revertants were found in the case of su+3 A1. One type corresponding to the true revertant A1 leads to G, and the other to the second site revertants C81 leads to U (A1U81). The A1U81 revertant has been shown to insert both glutamine and tyrosine at the site of amber mutation. Only true revertants (G82 leads to A) were obtained when su+3 G82 was analyzed. No revertants were obtained in the case of the su+3 A1G82. These results are discussed in relation to aminoacyl-tRNA recognition.

Full text

PDF
2799

Images in this article

2805Image
on p.2805
2805Image
on p.2805

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abelson J. N., Gefter M. L., Barnett L., Landy A., Russell R. L., Smith J. D. Mutant tyrosine transfer ribonucleic acids. J Mol Biol. 1970 Jan 14;47(1):15–28. doi: 10.1016/0022-2836(70)90398-0. [DOI] [PubMed] [Google Scholar]
  2. Altman S., Smith J. D. Tyrosine tRNA precursor molecule polynucleotide sequence. Nat New Biol. 1971 Sep 8;233(36):35–39. doi: 10.1038/newbio233035a0. [DOI] [PubMed] [Google Scholar]
  3. Brownlee G. G., Sanger F. Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli. J Mol Biol. 1967 Feb 14;23(3):337–353. doi: 10.1016/s0022-2836(67)80109-8. [DOI] [PubMed] [Google Scholar]
  4. Celis J. E., Coulondre C., Miller J. H. Suppressor su+7 inserts tryptophan in addition to glutamine. J Mol Biol. 1976 Jul 5;104(3):729–734. doi: 10.1016/0022-2836(76)90132-7. [DOI] [PubMed] [Google Scholar]
  5. Celis J. E., Smith J. D., Brenner S. Correlation between genetic and translational maps of gene 23 in bacteriophage T4. Nat New Biol. 1973 Jan 31;241(109):130–132. doi: 10.1038/newbio241130a0. [DOI] [PubMed] [Google Scholar]
  6. Crothers D. M., Seno T., Söll G. Is there a discriminator site in transfer RNA? Proc Natl Acad Sci U S A. 1972 Oct;69(10):3063–3067. doi: 10.1073/pnas.69.10.3063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ghysen A., Celis J. E. Mischarging single and double mutants of Escherichia coli sup3 tyrosine transfer RNA. J Mol Biol. 1974 Mar;83(3):333–351. doi: 10.1016/0022-2836(74)90283-6. [DOI] [PubMed] [Google Scholar]
  8. Inokuchi H., Celis J. E., Smith J. D. Letter: Mutant tyrosine transfer ribonucleic acids of Escherichia coli: construction by recombination of a double mutant A1G82 chargeable with glutamine. J Mol Biol. 1974 May 5;85(1):187–192. doi: 10.1016/0022-2836(74)90138-7. [DOI] [PubMed] [Google Scholar]
  9. Landy A., Abelson J., Goodman H. M., Smith J. D. Specific hybridization of tyrosine transfer ribonucleic acids with DNA from a transducing bacteriophage phi-80 carrying the amber suppressor gene su 3. J Mol Biol. 1967 Nov 14;29(3):457–471. doi: 10.1016/0022-2836(67)90112-x. [DOI] [PubMed] [Google Scholar]
  10. Russell R. L., Abelson J. N., Landy A., Gefter M. L., Brenner S., Smith J. D. Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol. 1970 Jan 14;47(1):1–13. doi: 10.1016/0022-2836(70)90397-9. [DOI] [PubMed] [Google Scholar]
  11. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  12. Smith J. D., Barnett L., Brenner S., Russell R. L. More mutant tyrosine transfer ribonucleic acids. J Mol Biol. 1970 Nov 28;54(1):1–14. doi: 10.1016/0022-2836(70)90442-0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES