Abstract
It has been determined previously that the protonation of the GC pairs induces a DNA conformation change which leads to a "metastable" structure. The role of the AT pairs, however, is no well known because the protonation does not modify their spectral properties. By means of an indirect method based on the binding of proflavine, it has been determined that the AT pairs are protonated before the acid-induced denaturation and that they seem to be unable to assume a conformation change when protonated. These results would indicate that the protonated AT pairs may be responsible for the induction of the acid denaturation and not the GC pairs as it was thought previously.
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Selected References
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- Courtois Y., Fromageot P., Guschlbauer W. Protonated polynucleotide structures. 3. An optical rotatory dispersion study of the protonation of DNA. Eur J Biochem. 1968 Dec 5;6(4):493–501. doi: 10.1111/j.1432-1033.1968.tb00472.x. [DOI] [PubMed] [Google Scholar]
- Fredericq E., Houssier C. Study of the interaction of DNA and acridine orange by various optical methods. Biopolymers. 1972;11(11):2281–2308. doi: 10.1002/bip.1972.360111108. [DOI] [PubMed] [Google Scholar]
- Guschlbauer W., Courtois Y. pH induced changes in optical activity of guanine nucleosides. FEBS Lett. 1968 Aug;1(3):183–186. doi: 10.1016/0014-5793(68)80055-9. [DOI] [PubMed] [Google Scholar]
- Lee C. H., Chang C. T., Wetmur J. G. Induced circular dichroism of DNA-dye complexes. Biopolymers. 1973 May;12(5):1098–1122. doi: 10.1002/bip.1973.360120514. [DOI] [PubMed] [Google Scholar]
- Ramstein J., Leng M. Interaction between proflavine and chemically methylated deoxyribonucleic acid. Biochim Biophys Acta. 1972 Sep 29;281(1):18–32. doi: 10.1016/0005-2787(72)90184-0. [DOI] [PubMed] [Google Scholar]
- Son T. D., Guschlbauer W., Guéron M. Flexibility and conformations of guanosine monophosphates by the Overhauser effect. J Am Chem Soc. 1972 Nov 1;94(22):7903–7911. doi: 10.1021/ja00777a038. [DOI] [PubMed] [Google Scholar]
- ZIMMER C., VENNER H. UNTERSUCHUNGEN AN NUCLEINSAEUREN. V. UBER DIE PH-ABHAENGIGKEIT DER THERMISCHEN DENATURIERUNG DER DESOXYRIBONUCLEINSAEUREN VERSCHIEDENEN GC-GEHALTES. J Mol Biol. 1963 Dec;7:603–609. doi: 10.1016/s0022-2836(63)80107-2. [DOI] [PubMed] [Google Scholar]