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. 1977 Sep;4(9):3039–3054. doi: 10.1093/nar/4.9.3039

Studies on gene control regions. VI. The 5- methyl of thymine, a lac repressor recognition site.

D V Goeddel, D G Yansura, M H Caruthers
PMCID: PMC342633  PMID: 333399

Abstract

Three site specific deoxyuridine analogs of lac operator were tested for binding with wild type (SQ) and tight binding (QX86) lac repressors. Insertion of uracil for thymine at site 13 (our nomenclature) significantly reduced the dissociation half-life of QX86 repressor for lac operator DNA (21 vs 1.2 min). Two other sites (6 and 7) are affected to a much lesser extent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bahl C. P., Wu R., Stawinsky J., Narang S. A. Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor. Proc Natl Acad Sci U S A. 1977 Mar;74(3):966–970. doi: 10.1073/pnas.74.3.966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Betz J. L., Sadler J. R. Tight-binding repressors of the lactose operon. J Mol Biol. 1976 Aug 5;105(2):293–319. doi: 10.1016/0022-2836(76)90113-3. [DOI] [PubMed] [Google Scholar]
  3. Gilbert W., Maizels N., Maxam A. Sequences of controlling regions of the lactose operon. Cold Spring Harb Symp Quant Biol. 1974;38:845–855. doi: 10.1101/sqb.1974.038.01.087. [DOI] [PubMed] [Google Scholar]
  4. Gill S. J., Wadsö I. An equation of state describing hydrophobic interactions. Proc Natl Acad Sci U S A. 1976 Sep;73(9):2955–2958. doi: 10.1073/pnas.73.9.2955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jobe A., Sadler J. R., Bourgeois S. lac Repressor-operator interaction. IX. The binding of lac repressor to operators containing Oc mutations. J Mol Biol. 1974 May 15;85(2):231–248. doi: 10.1016/0022-2836(74)90362-3. [DOI] [PubMed] [Google Scholar]
  6. Jovin T. M., Englund P. T., Bertsch L. L. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. J Biol Chem. 1969 Jun 10;244(11):2996–3008. [PubMed] [Google Scholar]
  7. Kleppe K., Ohtsuka E., Kleppe R., Molineux I., Khorana H. G. Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA's as catalyzed by DNA polymerases. J Mol Biol. 1971 Mar 14;56(2):341–361. doi: 10.1016/0022-2836(71)90469-4. [DOI] [PubMed] [Google Scholar]
  8. Lin S. Y., Riggs A. D. Lac repressor binding to operator analogues: comparison of poly(d(A-T)), poly(d(A-BrU)), and poly(d(A-U)). Biochem Biophys Res Commun. 1971 Dec 17;45(6):1542–1547. doi: 10.1016/0006-291x(71)90195-1. [DOI] [PubMed] [Google Scholar]
  9. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  10. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Panet A., van de Sande J. H., Loewen P. C., Khorana H. G., Raae A. J., Lillehaug J. R., Kleppe K. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry. 1973 Dec 4;12(25):5045–5050. doi: 10.1021/bi00749a003. [DOI] [PubMed] [Google Scholar]
  12. Riggs A. D., Bourgeois S., Cohn M. The lac repressor-operator interaction. 3. Kinetic studies. J Mol Biol. 1970 Nov 14;53(3):401–417. doi: 10.1016/0022-2836(70)90074-4. [DOI] [PubMed] [Google Scholar]
  13. Riggs A. D., Suzuki H., Bourgeois S. Lac repressor-operator interaction. I. Equilibrium studies. J Mol Biol. 1970 Feb 28;48(1):67–83. doi: 10.1016/0022-2836(70)90219-6. [DOI] [PubMed] [Google Scholar]
  14. Sanger F., Donelson J. E., Coulson A. R., Kössel H., Fischer D. Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1209–1213. doi: 10.1073/pnas.70.4.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Van de Sande J. H., Loewen P. C., Khorana H. G. Studies on polynucleotides. 118. A further study of ribonucleotide incorporation into deoxyribonucleic acid chains by deoxyribonucleic acid polymerase I of Escherichia coli. J Biol Chem. 1972 Oct 10;247(19):6140–6148. [PubMed] [Google Scholar]
  16. Weber H., Khorana H. G. CIV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxynucleotide corresponding to the nucleotide sequence 21 to 40. J Mol Biol. 1972 Dec 28;72(2):219–249. doi: 10.1016/0022-2836(72)90147-7. [DOI] [PubMed] [Google Scholar]
  17. Wells R. D., Blakesley R. W., Hardies S. C., Horn G. T., Larson J. E., Selsing E., Burd J. F., Chan H. W., Dodgson J. B., Jensen K. F. The role of DNA structure in genetic regulation. CRC Crit Rev Biochem. 1977;4(3):305–340. doi: 10.3109/10409237709102561. [DOI] [PubMed] [Google Scholar]
  18. Yansura D. G., Goeddel D. V., Caruthers M. H. Studies on gene control regions. 2. Enzymatic joining of chemically synthesized lactose operator deoxyribonucleic acid segments. Biochemistry. 1977 May 3;16(9):1772–1780. doi: 10.1021/bi00628a002. [DOI] [PubMed] [Google Scholar]
  19. Yansura D. G., Goeddel D. V., Cribbs D. L., Caruthers M. H. Studies of gene control regions. III. Binding of synthetic and modified synthetic lac operator DNAs to lactose repressor. Nucleic Acids Res. 1977 Mar;4(3):723–737. doi: 10.1093/nar/4.3.723. [DOI] [PMC free article] [PubMed] [Google Scholar]

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