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. 1977 Sep;4(9):3083–3095. doi: 10.1093/nar/4.9.3083

DNA synthesis by partially purified replicating simian virus 40 chromosomes.

H J Edenberg, M A Waqar, J A Huberman
PMCID: PMC342636  PMID: 198750

Abstract

We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Replicating SV40 chromosomes isolated from such gradients are capable of continuing DNA synthesis in vitro in the same manner as two crude subnuclear systems we have previously described (4). This indicates that the enzymes and other proteins required for in vitro DNA synthesis are bound to the replicating chromosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cremisi C., Pignatti P. F., Croissant O., Yaniv M. Chromatin-like structures in polyoma virus and simian virus 10 lytic cycle. J Virol. 1975 Jan;17(1):204–211. doi: 10.1128/jvi.17.1.204-211.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DePamphilis M. L., Berg P. Requirement of a Cytoplasmic Fraction for Synthesis of SV40 Deoxyribonucleic Acid in Isolated Nuclei*. J Biol Chem. 1975 Jun 10;250(11):4348–4354. [PubMed] [Google Scholar]
  3. Edenberg H. J., Huberman J. A. Eukaryotic chromosome replication. Annu Rev Genet. 1975;9:245–284. doi: 10.1146/annurev.ge.09.120175.001333. [DOI] [PubMed] [Google Scholar]
  4. Edenberg H. J., Waqar M. A., Huberman J. A. Subnuclear systems for synthesis of simian virus 40 DNA in vitro. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4392–4396. doi: 10.1073/pnas.73.12.4392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Francke B., Hunter T. In vitro polyoma DNA synthesis: requirement for cytoplasmic factors. J Virol. 1975 Jan;15(1):97–107. doi: 10.1128/jvi.15.1.97-107.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldstein D. A., Hall M. R., Meinke W. Properties of nucleoprotein complexes containing replicating polyoma DNA. J Virol. 1973 Oct;12(4):887–900. doi: 10.1128/jvi.12.4.887-900.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Green M. H. Biosynthetic properties of a polyoma nucleoprotein complex: evidence for replication sites. J Virol. 1972 Jul;10(1):32–41. doi: 10.1128/jvi.10.1.32-41.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Griffith J. D. Chromatin structure: deduced from a minichromosome. Science. 1975 Mar 28;187(4182):1202–1203. doi: 10.1126/science.187.4182.1202. [DOI] [PubMed] [Google Scholar]
  9. Hall M. R., Meinke W., Goldstein D. A. Nucleoprotein complexes containing replicating Simian virus 40 DNA: comparison with polyoma nucleoprotein complexes. J Virol. 1973 Oct;12(4):901–908. doi: 10.1128/jvi.12.4.901-908.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hershey H. V., Stieber J. F., Mueller G. C. Dna synthesis in isolated HeLa nuclei. A system for continuation of replication in vivo. Eur J Biochem. 1973 Apr;34(2):383–394. doi: 10.1111/j.1432-1033.1973.tb02770.x. [DOI] [PubMed] [Google Scholar]
  11. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  12. Otto B., Reichard P. Replication of polyoma DNA in isolated nuclei. V. Complementation of in vitro DNA replication. J Virol. 1975 Feb;15(2):259–267. doi: 10.1128/jvi.15.2.259-267.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Seale R. L. Studies on the mode of segregation of histone nu bodies during replication in HeLa cells. Cell. 1976 Nov;9(3):423–429. doi: 10.1016/0092-8674(76)90087-8. [DOI] [PubMed] [Google Scholar]
  14. Seebeck T., Weil R. Polyoma viral DNA replicated as a nucleoprotein complex in close association with the host cell chromatin. J Virol. 1974 Mar;13(3):567–576. doi: 10.1128/jvi.13.3.567-576.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Seki S., Mueller G. C. Dissociation and reconstitution of the DNA replicase system of HeLa cell nuclei. Biochim Biophys Acta. 1976 Jul 2;435(3):236–250. doi: 10.1016/0005-2787(76)90105-2. [DOI] [PubMed] [Google Scholar]
  16. Su R. T., DePamphilis M. L. In vitro replication of simian virus 40 DNA in a nucleoprotein complex. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3466–3470. doi: 10.1073/pnas.73.10.3466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tegtmeyer P. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972 Oct;10(4):591–598. doi: 10.1128/jvi.10.4.591-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weintraub H. The assembly of newly replicated DNA into chromatin. Cold Spring Harb Symp Quant Biol. 1974;38:247–256. doi: 10.1101/sqb.1974.038.01.028. [DOI] [PubMed] [Google Scholar]
  19. White M., Eason R. Nucleoprotein complexes in simian virus 40-infected cells. J Virol. 1971 Oct;8(4):363–371. doi: 10.1128/jvi.8.4.363-371.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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