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. 2012 Mar;1(1):13–24. doi: 10.1002/mbo3.3

Figure 4.

Figure 4

Analyses of lcp disruption mutants of Streptomyces sp. K30. (a) Screening for lcp disruption mutants of Streptomyces sp. strain K30 by colony PCR. Cell material from a single colony of a putative lcp disruption mutant was suspended in 50-μl TE buffer, and the suspension was then boiled for 15 min. After centrifugation, 0.5 μl was applied as template for a PCR employing the primers N_Lcp and C_Lcp (Table 1); the product was subsequently separated in a 1% (w/v) agarose gel. M, λ DNA digested with Pst I; WT, Streptomyces sp. K30 wild type; lcp ΩKm disruption mutant of Streptomyces sp. K30. (b and c) Effect of the knock out lcp mutant on clear zone formation by Streptomyces sp. K30. (b)Streptomyces sp. K30 harboring lcp ΩKm and (c) the wild-type Streptomyces sp. K30 were cultivated for seven days on a natural rubber (NR) latex overlay agar plate at 30°C.