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. 1977 Oct;4(10):3599–3615. doi: 10.1093/nar/4.10.3599

An NMR study of the exchange rates for protons involved in the secondary and tertiary structure of yeast tRNA Phe.

P D Johnston, A G Redfield
PMCID: PMC342676  PMID: 337239

Abstract

Solvent exchange rates of all the protons of yeast tRNAphe resonating in the lowfield NMR region (-11 to-15 ppm from DSS) have been measured by saturation-recovery long-pulse Fourier transform NMR. All these protons in yeast tRNAphe are in the fast exchange limit with H2O relative to their intrinsic longitudinal relaxation processes. Most rates show very little temperature dependence; however, tertiary base pair protons are preferentially destabilized in the absence of Mg++ at higher temperatures. The measured exchange rates are between 2 and 125 sec-1 for a temperature range from 10 degrees C to 45 degrees C and MgCl2 concentrations between 0 and 15 mM.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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