Abstract
Protein L24, from the Escherichia coli ribosome, protects a large region of 23S RNA against ribonuclease digestion. This protected RNA consists of a series of non-contiguous subfragments encompassing about 480 nucleotides at the 5' -end of 23S RNA (1). The present work demonstrates that this RNA moiety remains intact, after removal of protein L24, indicating that the subfragments are maintained together by RNA-RNA interactions. Using a urea washing procedure, the weakly bound RNA subfragments were selectively removed leaving more strongly interacting subfragments that were identified, by gel electrophoresis and oligonucleotide fingerprinting, and shown to derive from widely separated sequence regions.
Full text
PDF










Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Branlant C., Korobko V., Ebel J. P. The binding site of protein L1 on 23-S ribosomal RNA from Escherichia coli. 3. Nucleotide sequence. Eur J Biochem. 1976 Nov 15;70(2):471–482. doi: 10.1111/j.1432-1033.1976.tb11038.x. [DOI] [PubMed] [Google Scholar]
- Branlant C., Sri Widada J., Krol A., Ebel J. P. RNA sequences in ribonucleoprotein fragments of the complex formed from ribosomal 23-S RNA and ribosomal protein L24 of Escherichia coli. Eur J Biochem. 1977 Mar 15;74(1):155–170. doi: 10.1111/j.1432-1033.1977.tb11377.x. [DOI] [PubMed] [Google Scholar]
- Garrett R. A., Rak K. H., Daya L., Stöffler G. Ribosomal proteins. XXIX. Specific protein binding sites on 16S rRNA of Escherichia coli. Mol Gen Genet. 1972;114(2):112–124. doi: 10.1007/BF00332782. [DOI] [PubMed] [Google Scholar]
- Garrett R. A., Ungewickell E., Newberry V., Hunter J., Wagner R. An RNA core in the 30S ribosomal subunit of Escherichia coli and its structural and functional significance. Cell Biol Int Rep. 1977 Nov;1(6):487–502. doi: 10.1016/0309-1651(77)90086-8. [DOI] [PubMed] [Google Scholar]
- Mackie G. A., Zimmermann R. A. Characterization of fragments of 16 S ribonucleic acid protected against pancreatic ribonuclease digestion by ribosomal protein S4. J Biol Chem. 1975 Jun 10;250(11):4100–4112. [PubMed] [Google Scholar]
- Nanninga N., Garrett R. A., Stöffler G., Klotz G. Ribosomal proteins. 38. Electron microscopy of ribosomal protein S4-16S RNA complexes of Escherichia coli. Mol Gen Genet. 1972;119(2):175–184. doi: 10.1007/BF00269135. [DOI] [PubMed] [Google Scholar]
- Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
- Sloof P., Garrett R. A., Nanninga N. An investigation of the binding sites of proteins S8, L23 and L24 on the ribosomal RNAs of Escherichia coli by electron microscopy. Mol Gen Genet. 1976 Aug 19;147(2):129–138. doi: 10.1007/BF00267564. [DOI] [PubMed] [Google Scholar]
- Tritton T. R., Crothers D. M. Physical characterization of a ribosomal nucleoprotein complex. Biochemistry. 1976 Oct 5;15(20):4377–4385. doi: 10.1021/bi00665a005. [DOI] [PubMed] [Google Scholar]
- Ungewickell E., Ehresmann C., Stiegler P., Garrett R. Evidence for tertiary structural RNA-RNA interactions within the protein S4 binding site at the 5'-end of 16S ribosomal RNA of Escherichia coli.+. Nucleic Acids Res. 1975 Oct;2(10):1867–1888. doi: 10.1093/nar/2.10.1867. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ungewickell E., Garrett R. A. Further characterisation of the RNA structure in the binding region of protein S4 on 16 S ribosomal RNA of Escherichia coli. FEBS Lett. 1977 Sep 1;81(1):193–198. doi: 10.1016/0014-5793(77)80957-5. [DOI] [PubMed] [Google Scholar]