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. 1978 Oct;5(10):3549–3563. doi: 10.1093/nar/5.10.3549

Specific termination of RNA polymerase synthesis as a method of RNA and DNA sequencing.

V D Axelrod, R M Vartikyan, V A Aivazashvili, R S Beabealashvili
PMCID: PMC342694  PMID: 724495

Abstract

Termination of RNA synthesis with 3'-O-Methylnucleoside 5'-triphosphates have been studied using E. coli RNA polymerase holoenzyme and poly [d(A-T)] as well as unfractionated T7 D delta III DNA as templates. It was shown that the termination can be used for DNA sequencing. A sequence of a part of RNA synthesized from AI promoter of the DNA have been determined. Syntheses of four 3'-O-Methylnucleoside 5'-triphosphates are described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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