Abstract
The interaction of N--Acetyl--Phe--tRNA Phe with 70 S ribosomes is a reversible process in the absence as well as in the presence of messenger. The equilibrium binding constants of these interactions were measured at different magnesium concentrations and temperatures and thermodynamical quantities computed. The enthalpy of the formation of complexes with the P site of ribosomes is larger by 6,000 cal/mol in the presence of poly (U) than in the presence of poly (C) or in total absence of messenger. Free energy differences are rather small, the association constants differ less than one order of magnitude. The association constant of N--Acetyl--Phe--tRNA Phe with the A site of ribosomes is 30--50 times lower than with the P site even in the presence of poly (U).
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Selected References
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- Bresler S. E., Vlasov G. P., Kirillov S. V., Semenkov Iu P., Makhno V. I. Nefermentativnoe spetsificheskoe (kodon-zavisimoe) sviazyvanie tRNK s izolirovannoi 30S ribosomnoi sub'edinitsei. Izmerenie konstant dissotsiatsii kompleksa. Dokl Akad Nauk SSSR. 1975 Jan 21;220(3):719–722. [PubMed] [Google Scholar]
- Leder P. The elongation reactions in protein synthesis. Adv Protein Chem. 1973;27:213–242. doi: 10.1016/s0065-3233(08)60448-9. [DOI] [PubMed] [Google Scholar]
- NIRENBERG M., LEDER P. RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES. Science. 1964 Sep 25;145(3639):1399–1407. doi: 10.1126/science.145.3639.1399. [DOI] [PubMed] [Google Scholar]
- Rappoport S., Lapidot Y. The chemical preparation of acetylaminoacyl-tRNA. Methods Enzymol. 1974;29:685–688. doi: 10.1016/0076-6879(74)29060-8. [DOI] [PubMed] [Google Scholar]