Table 3. Conditional Logistic Regression analysis of global DNA methylation biomarkers and breast cancer risk (pooled and by source), New York site of the BCFR.
| DNA Methylation | Odds Ratios and 95% Confidence Intervals for Overall Pooled sample and Stratified by Sourcea |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
[3H]-methyl acceptance assayb |
|
Pooled (n = 458) |
|
Granulocytes (n = 156) |
|
Total WBC (n = 302) |
||||
| OR |
95% CI |
OR |
95% CI |
OR |
95% CI |
|||||
| Quartile (DPM/μg DNA) |
Q4 (> 115215); lower overall methylation |
1.81 |
0.99–3.32 |
2.42 |
0.86–6.83 |
1.51 |
0.68–3.38 |
|||
| Q3 (68940 – 115215) |
1.81 |
1.02–3.20 |
1.34 |
0.46–3.87 |
1.76 |
0.84–3.67 |
||||
| Q2 (41692 – 68940) |
0.82 |
0.46–1.46 |
0.91 |
0.42–2.00 |
0.67 |
0.28–1.61 |
||||
| Q1 (≤ 41692); higher overall methylation |
1 |
|
1 |
|
1 |
|
||||
| Continuous (log DPM/μg DNA) |
1 unit change, less methylation |
1.34 |
1.03–1.74 |
1.18 |
0.82–1.70 |
1.49 |
1.03–2.16 |
|||
| | ||||||||||
| LUMAc | |
Pooled (n = 551) |
Granulocytes (n = 201) |
Total WBC (n = 350) |
||||||
| OR |
95% CI |
OR |
95% CI |
OR |
95% CI |
|||||
| Quartile (DNA meth %) |
Q1 (≤ 62.9), lower overall methylation |
0.92 |
0.53–1.59 |
1.18 |
0.43–3.24 |
0.8 |
0.41–1.55 |
|||
| Q2 (62.9 - 68.0) |
0.71 |
0.40–1.24 |
1.12 |
0.39–3.21 |
0.58 |
0.29–1.14 |
||||
| Q3 (68.0 - 73.0) |
1.26 |
0.75–2.12 |
1.9 |
0.79–4.55 |
0.98 |
0.51–1.91 |
||||
| Q4 (> 73.0); higher overall methylation |
1 |
|
1 |
|
1 |
|
||||
| Continuous | 1 unit change, less methylation | 1.00 | 0.97–1.02 | 1.02 | 0.97–1.07 | 0.99 | 0.96–1.02 | |||
a OR = odds ratio, 95% CI = 95% confidence intervals; adjusted for age at blood draw and smoking status. bHigher values on assay mean lower DNA methylation. cLower values on assay mean lower DNA methylation