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. 1978 Nov;5(11):4055–4064. doi: 10.1093/nar/5.11.4055

Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae.

J Tomassini, R Roychoudhury, R Wu, R J Roberts
PMCID: PMC342733  PMID: 82935

Abstract

We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV40) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).

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Selected References

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