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. 1978 Nov;5(11):4317–4328. doi: 10.1093/nar/5.11.4317

DNA ligase activity in UV-irradiated monkey kidney cells.

M Mezzina, S Nocentini
PMCID: PMC342751  PMID: 724516

Abstract

The DNA ligase activity of monkey kidney CV-1 cells has been measured at different stages of culture growth and after different time intervals following ultraviolet irradiation. Results indicate that: - The level of enzyme activity is about twice higher in non synchronous, rapidly dividing cells than in confluent cultures. - UV-irradiation of cells induces a "de novo" synthesis of DNA ligase. - This induction is dose dependent in its extent and kinetics, and may lead to a DNA ligase level in UV-irradiated stationary cultures of the same order as observed in unirradiated exponentially growing cells. - This induction seems to be independent of semiconservative DNA synthesis since it is not affected by fluorodeoxyuridine.

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Selected References

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