Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1978 Dec;5(12):4563–4578. doi: 10.1093/nar/5.12.4563

A conserved and unique (AT)-rich segment in yeast mitochondrial DNA.

J L Bos, C F Van Kreijl, F H Ploegaert, J N Mol, P Borst
PMCID: PMC342773  PMID: 370773

Abstract

The mtDNA of the cytoplasmic petite mutant of yeast RD1A consists mainly of a perfect head-to-tail repetition of a known sequence of 66 consecutive AT and 2 GC base pairs. We have hybridized complementary RNA made on RD1A mtDNA with the mtDNAs of four different wild-type Saccharomyces strains that differ markedly in restriction fragmentation pattern. The tm's of the four heteroduplexes are identical to the tm of the homoduplex of RD1A mtDNA with complementary RNA of one repeat length. With all four wild-type mtDNAs this complementary RNA hybridizes mainly to a single restriction fragment of about 300 base pairs. This shows the conservation and individuality of at least one (AT)-rich segment in yeast mtDNA. The 300 base pair fragment has been mapped in the vicinity of the oxi-2 locus. The possible role of the (AT)-rich segment in the processing of the primary transcript of this region is discussed.

Full text

PDF
4563

Images in this article

4571Image
on p.4571
4573Image
on p.4573
4575Image
on p.4575

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernardi G., Faures M., Piperno G., Slonimski P. P. Mitochondrial DNA's from respiratory-sufficient and cytoplasmic respiratory-deficient mutant yeast. J Mol Biol. 1970 Feb 28;48(1):23–42. doi: 10.1016/0022-2836(70)90216-0. [DOI] [PubMed] [Google Scholar]
  2. Bernardi G., Piperno G., Fonty G. The mitochondrial genome of wild-type yeast cells. I. Preparation and heterogeneity of mitochondrial DNA. J Mol Biol. 1972 Mar 28;65(2):173–189. doi: 10.1016/0022-2836(72)90275-6. [DOI] [PubMed] [Google Scholar]
  3. Borst P., Grivell L. A. The mitochondrial genome of yeast. Cell. 1978 Nov;15(3):705–723. doi: 10.1016/0092-8674(78)90257-x. [DOI] [PubMed] [Google Scholar]
  4. Bos J. L., Heyting C., Borst P., Arnberg A. C., Van Bruggen E. F. An insert in the single gene for the large ribosomal RNA in yeast mitochondrial DNA. Nature. 1978 Sep 28;275(5678):336–338. doi: 10.1038/275336a0. [DOI] [PubMed] [Google Scholar]
  5. Faye G., Fukuhara H., Grandchamp C., Lazowska J., Michel F., Casey J., Getz G. S., Locker J., Rabinowitz M., Bolotin-Fukuhara M. Mitochondrial nucleic acids in the petite colonie mutants: deletions and repetition of genes. Biochimie. 1973;55(6):779–792. doi: 10.1016/s0300-9084(73)80030-6. [DOI] [PubMed] [Google Scholar]
  6. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  7. Gralla J., Crothers D. M. Free energy of imperfect nucleic acid helices. 3. Small internal loops resulting from mismatches. J Mol Biol. 1973 Aug 5;78(2):301–319. doi: 10.1016/0022-2836(73)90118-6. [DOI] [PubMed] [Google Scholar]
  8. Grossman L. I., Cryer D. R., Goldring E. S., Marmur J. The petite mutation in yeast. 3. Nearest-neighbor analysis of mitochondrial DNA from normal and mutant cells. J Mol Biol. 1971 Dec 28;62(3):565–575. doi: 10.1016/0022-2836(71)90156-2. [DOI] [PubMed] [Google Scholar]
  9. Hollenberg C. P., Borst P., Flavell R. A., van Kreijl C. F., van Bruggen E. F., Arnberg A. C. The unusual properties of mtDNA from a "low-density" petite mutant of yeast. Biochim Biophys Acta. 1972 Aug 16;277(1):44–58. doi: 10.1016/0005-2787(72)90350-4. [DOI] [PubMed] [Google Scholar]
  10. Hutton J. R., Wetmur J. G. Effect of chemical modification on the rate of renaturation of deoxyribonucleic acid. Deaminated and glyoxalated deoxyribonucleic acid. Biochemistry. 1973 Jan 30;12(3):558–563. doi: 10.1021/bi00727a032. [DOI] [PubMed] [Google Scholar]
  11. Lomant A. J., Fresco J. R. Structural and energetic consequences of noncomplementary base oppositions in nucleic acid helices. Prog Nucleic Acid Res Mol Biol. 1975;15(0):185–218. doi: 10.1016/s0079-6603(08)60120-8. [DOI] [PubMed] [Google Scholar]
  12. Mol J. N., Borst P., Grosveld F. G., Spencer J. H. The size of the repeating unit of the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast. Biochim Biophys Acta. 1974 Dec 6;374(2):115–128. doi: 10.1016/0005-2787(74)90355-4. [DOI] [PubMed] [Google Scholar]
  13. Piperno G., Fonty G., Bernardi G. The mitochondrial genome of wild-type yeast cells. II. Investigations on the compositional heterogeneity of mitochondrial DNA. J Mol Biol. 1972 Mar 28;65(2):191–205. doi: 10.1016/0022-2836(72)90276-8. [DOI] [PubMed] [Google Scholar]
  14. Prunell A., Bernardi G. The mitochondrial genome of wild-type yeast cells. IV. Genes and spacers. J Mol Biol. 1974 Jul 15;86(4):825–841. doi: 10.1016/0022-2836(74)90356-8. [DOI] [PubMed] [Google Scholar]
  15. Prunell A., Bernardi G. The mitochondrial genome of wild-type yeast cells. VI. Genome organization. J Mol Biol. 1977 Feb 15;110(1):53–74. doi: 10.1016/s0022-2836(77)80098-3. [DOI] [PubMed] [Google Scholar]
  16. Prunell A., Kopecka H., Strauss F., Bernardi G. The mitochondrial genome of wild-type yeast cells. V. Genome evolution. J Mol Biol. 1977 Feb 15;110(1):17–47. doi: 10.1016/s0022-2836(77)80096-x. [DOI] [PubMed] [Google Scholar]
  17. Riley M., Maling B. Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes. J Mol Biol. 1966 Sep;20(2):359–389. doi: 10.1016/0022-2836(66)90069-6. [DOI] [PubMed] [Google Scholar]
  18. Sanders J. P., Weijers P. J., Groot G. S., Borst P. Properties of mitochondrial DNA from Kluyveromyces lactis. Biochim Biophys Acta. 1974 Dec 6;374(2):136–144. doi: 10.1016/0005-2787(74)90357-8. [DOI] [PubMed] [Google Scholar]
  19. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. Ullman J. S., McCarthy B. J. The relationship between mismatched base pairs and the thermal stability of DNA duplexes. II. Effects of deamination of cytosine. Biochim Biophys Acta. 1973 Feb 4;294(1):416–424. doi: 10.1016/0005-2787(73)90096-8. [DOI] [PubMed] [Google Scholar]
  22. Van Kreijl C. F., Bos J. L. The repeating nucleotide sequence in the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast. Nucleic Acids Res. 1977 Jul;4(7):2369–2388. doi: 10.1093/nar/4.7.2369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Van Ommen G. J., Groot G. S., Borst P. Fine structure physical mapping of 4S RNA genes on mitochondrial DNA of Saccharomyces cerevisiae. Mol Gen Genet. 1977 Sep 9;154(3):255–262. doi: 10.1007/BF00571280. [DOI] [PubMed] [Google Scholar]
  24. Wilson D. A., Thomas C. A., Jr Hydroxyapatite chromatography of short double-helical DNA. Biochim Biophys Acta. 1973 Dec 21;331(3):333–340. doi: 10.1016/0005-2787(73)90019-1. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES