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. 1978 Dec;5(12):4795–4803. doi: 10.1093/nar/5.12.4795

Caffeine inhibits excision of 7-bromomethylbenz (a) anthracene-DNA adducts from exponentially growing but not from stationary phase Chinese hamster cells.

F Friedlos, J J Roberts
PMCID: PMC342789  PMID: 745993

Abstract

Excision of 7-bromomethylbenz(a)anthracene (7-BMBA)-DNA adducts from exponentially growing cultures of Chinese hamster V79-379A cells followed logarithmic kinetics with a half of approximately 20 hrs. Post-treatment incubation in the presence of a sub-toxic concentration of caffeine markedly reduced this loss. Caffeine brought about a concomitant increase in overall DNA synthetic rate in treated exponential cultures. Excision in stationary, non-DNA-replicating cultures, was slower and caffeine did not affect this reduced rate of excision. These findings lend support to a previous proposition that the caffeine-induced inhibition of elongation of nascent DNA on a template containing chemical lesions results in an interference with the excision repair mechanism that removes these lesions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyd J. B., Presley J. M. Repair replication and photorepair of DNA in larvae of Drosophila melanogaster. Genetics. 1974 Aug;77(4):687–700. doi: 10.1093/genetics/77.4.687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cleaver J. E. Repair replication of mammalian cell DNA: effects of compounds that inhibit DNA synthesis or dark repair. Radiat Res. 1969 Feb;37(2):334–348. [PubMed] [Google Scholar]
  3. Dipple A., Brookes P., Mackintosh D. S., Rayman M. P. Reaction of 7-bromomethylbenz(a)anthracene with nucleic acids, polynucleotides, and nucleosides. Biochemistry. 1971 Nov;10(23):4323–4330. doi: 10.1021/bi00799a026. [DOI] [PubMed] [Google Scholar]
  4. Dipple A., Roberts J. J. Excision of 7-bromomethylbenz[a]anthracene--DNA adducts in replicating mammalian cells. Biochemistry. 1977 Apr 5;16(7):1499–1503. doi: 10.1021/bi00626a039. [DOI] [PubMed] [Google Scholar]
  5. Dipple A., Shooter K. V. Chemical modification and biological inactivation of bacteriophage R17 by 7-bromomethylbenz(alpha)anthracenes. Biochim Biophys Acta. 1974 Dec 20;374(3):392–399. doi: 10.1016/0005-2787(74)90260-3. [DOI] [PubMed] [Google Scholar]
  6. Dipple A., Slade T. A. Structure and activity in chemical carcinogenesis: reactivity and carcinogenicity of 7-bromomethylbenz[alpha]anthracene and 7-bromomethyl-12-methylbenz[alpha]anthracene. Eur J Cancer. 1970 Oct;6(5):417–423. doi: 10.1016/0014-2964(70)90040-x. [DOI] [PubMed] [Google Scholar]
  7. Duncan M. E., Brookes P. The induction of azaguanine-resistant mutants in cultured Chinese hamster cells by reactive derivatives of carcinogenic hydrocarbons. Mutat Res. 1973 Apr;21(2):107–118. [PubMed] [Google Scholar]
  8. Friedlos F., Roberts J. J. Caffeine-inhibited DNA repair in 7-bromomethylbenz (a)-anthracene-treated Chinease hamster cells: formation of breaks in parental DNA and inhibition of ligation of nascent DNA as a mechanism for enhancement of lethality and chromosome damage. Mutat Res. 1978 May;50(2):263–275. doi: 10.1016/0027-5107(78)90030-1. [DOI] [PubMed] [Google Scholar]
  9. Gaudin D., Gregg R. S., Yielding K. L. Inhibition of DNA repair replication by DNA binding drugs which sensitize cells to alkylating agents and X-rays. Proc Soc Exp Biol Med. 1972 Nov;141(2):543–547. doi: 10.3181/00379727-141-36818. [DOI] [PubMed] [Google Scholar]
  10. Grigg G. W. Effects of coumarin, pyronin Y, 6,9-dimethyl 2-methylthiopurine and caffeine on excision repair and recombination repair in Escherichia coli. J Gen Microbiol. 1972 Apr;70(2):221–230. doi: 10.1099/00221287-70-2-221. [DOI] [PubMed] [Google Scholar]
  11. Huberman E., Aspiras L., Heidelberger C., Grover P. L., Sims P. Mutagenicity to mammalian cells of epoxides and other derivatives of polycyclic hydrocarbons. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3195–3199. doi: 10.1073/pnas.68.12.3195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kirby K. S., Cook E. A. Isolation of deoxyribonucleic acid from mammalian tissues. Biochem J. 1967 Jul;104(1):254–257. doi: 10.1042/bj1040254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lieberman M. W., Dipple A. Removal of bound carcinogen during DNA repair in nondividing human lymphocytes. Cancer Res. 1972 Sep;32(9):1855–1860. [PubMed] [Google Scholar]
  14. Maher V. M., Douville D., Tomura T., Van Lancker J. L. Mutagenicity of reactive derivatives of carcinogenic hydrocarbons: evidence of DNA repair. Mutat Res. 1974 Apr;23(1):113–128. doi: 10.1016/0027-5107(74)90166-3. [DOI] [PubMed] [Google Scholar]
  15. Roberts J. J., Friedlos F., Van Den Berg H. W., Kirkland D. J. Inhibition by caffeine of post-replication repair in Chinese hamster cells treated with 7-bromomethylbenz(a)anthracene: enhancement of toxicity, chromosome damage and inhibition of ligation of newly-synthesized DNA. Chem Biol Interact. 1977 Jun;17(3):265–290. doi: 10.1016/0009-2797(77)90091-6. [DOI] [PubMed] [Google Scholar]
  16. Roberts J. J., Pascoe J. M., Plant J. E., Sturrock J. E., Crathorn A. R. Quantitative aspects of the repair of alkylated DNA in cultured mammalian cells. I. The effect on HeLa and Chinese hamster cell survival of alkylation of cellular macromolecules. Chem Biol Interact. 1971 Feb;3(1):29–47. doi: 10.1016/0009-2797(71)90024-x. [DOI] [PubMed] [Google Scholar]
  17. Roberts J. J., Ward K. N. Inhibition of post-replication repair of alkylated DNA by caffeine in Chinese hamster cells but not HeLa cells. Chem Biol Interact. 1973 Oct;7(4):241–264. doi: 10.1016/0009-2797(73)90027-6. [DOI] [PubMed] [Google Scholar]
  18. Roe F. J., Dipple A., Mitchley B. C. Carcinogenic activity of some benz(a)anthracene derivatives in newborn mice. Br J Cancer. 1972 Dec;26(6):461–465. doi: 10.1038/bjc.1972.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Van Den Berg H. W., Roberts J. J. Post-replication repair of DNA in Chinese hamster cells treated with cis platinum (II) diamine dichloride. Enhancement of toxicity and chromosome damage by caffeine. Mutat Res. 1975 Dec;33(2-3):279–284. doi: 10.1016/0027-5107(75)90203-1. [DOI] [PubMed] [Google Scholar]

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