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. 1978 Dec;5(12):4865–4876. doi: 10.1093/nar/5.12.4865

The role of the guanine insertion enzyme in O-biosynthesis in Drosophila melanogaster.

R D McKinnon, M A Wosnick, B N White
PMCID: PMC342794  PMID: 106366

Abstract

Drosophila tRNA can be guanylated by a crude enzyme from rabbit reticulocytes. Guanylating activity is also present in crude extracts of adult Drosophila. A major product of this reaction as well as several minor ones were resolved by RPC-5 chromatography. The main substrate of both the Drosophila and rabbit reticulocyte enzymes was the non-Q-containing aspartic acid tRNA, tRNA2gammaAsp. The QU-lacking (gamma) forms of asparagine, histidine and tyrosine tRNAs were also substrates and gave rise to the minor products of the reaction. In contrast, the Q- or Q*-containing (delta) forms of these tRNAs appear not to be substrates. The evidence strongly suggests that the guanyating enzyme is involved in Q biosynthesis and would be better termed a guanine replacement or pre-Q insertion enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dubrul E. F., Farkas W. R. Partial purification and properties of the reticulocyte guanylating enzyme. Biochim Biophys Acta. 1976 Sep 6;442(3):379–390. doi: 10.1016/0005-2787(76)90312-9. [DOI] [PubMed] [Google Scholar]
  2. Farkas W. R., Chernoff D. Identification of the minor guanylated tRNA of rabbit reticulocytes. Nucleic Acids Res. 1976 Oct;3(10):2521–2528. doi: 10.1093/nar/3.10.2521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hankins W. D., Farkas W. R. Guanylation of transfer RNA by rabbit reticulocytes. Biochim Biophys Acta. 1970 Jul 16;213(1):77–89. doi: 10.1016/0005-2787(70)90009-2. [DOI] [PubMed] [Google Scholar]
  4. Harada F., Nishimura S. Possible anticodon sequences of tRNA His , tRNA Asm , and tRNA Asp from Escherichia coli B. Universal presence of nucleoside Q in the first postion of the anticondons of these transfer ribonucleic acids. Biochemistry. 1972 Jan 18;11(2):301–308. doi: 10.1021/bi00752a024. [DOI] [PubMed] [Google Scholar]
  5. KIRBY K. S. A new method for the isolation of ribonucleic acids from mammalian tissues. Biochem J. 1956 Nov;64(3):405–408. doi: 10.1042/bj0640405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kasai H., Kuchino Y., Nihei K., Nishimura S. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's. Nucleic Acids Res. 1975 Oct;2(10):1931–1939. doi: 10.1093/nar/2.10.1931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kasai H., Nakanishi K., Macfarlane R. D., Torgerson D. F., Ohashi Z., McCloskey J. A., Gross H. J., Nishimura S. Letter: The structure of Q* nucleoside isolated from rabbit liver transfer ribonucleic acid. J Am Chem Soc. 1976 Aug 4;98(16):5044–5046. doi: 10.1021/ja00432a071. [DOI] [PubMed] [Google Scholar]
  8. Kasai H., Oashi Z., Harada F., Nishimura S., Oppenheimer N. J., Crain P. F., Liehr J. G., von Minden D. L., McCloskey J. A. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine. Biochemistry. 1975 Sep 23;14(19):4198–4208. doi: 10.1021/bi00690a008. [DOI] [PubMed] [Google Scholar]
  9. Kelmers A. D., Novelli G. D., Stulberg M. P. Separation of transfer ribonucleic acids by reverse phase chromatography. J Biol Chem. 1965 Oct;240(10):3979–3983. [PubMed] [Google Scholar]
  10. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  11. Okada N., Harada F., Nishimura S. Specific replacement of Q base in the anticodon of tRNA by guanine catalyzed by a cell-free extract of rabbit reticulocytes. Nucleic Acids Res. 1976 Oct;3(10):2593–2603. doi: 10.1093/nar/3.10.2593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Okada N., Yasuda T., Nishimura S. Detection of nucleoside Q precursor in methyl-deficient E.coli tRNA. Nucleic Acids Res. 1977 Dec;4(12):4063–4075. doi: 10.1093/nar/4.12.4063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pearson R. L., Weiss J. F., Kelmers A. D. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns. Biochim Biophys Acta. 1971 Feb 11;228(3):770–774. doi: 10.1016/0005-2787(71)90748-9. [DOI] [PubMed] [Google Scholar]
  14. Suhadolnik R. J., Uematsu T. Biosynthesis of the pyrrolopyrimidine nucleoside antibiotic, toyocamycin. VII. Origin of the pyrrole carbons and the cyano carbon. J Biol Chem. 1970 Sep 10;245(17):4365–4371. [PubMed] [Google Scholar]
  15. Twardzik D. R., Grell E. H., Jacobson K. B. Mechanism of suppression in Drosophila: a change in tyrosine transfer RNA. J Mol Biol. 1971 Apr 28;57(2):231–245. doi: 10.1016/0022-2836(71)90343-3. [DOI] [PubMed] [Google Scholar]
  16. White B. N. Chromatographic changes in specific tRNAs after reaction with cyanogen bromide and sodium periodate. Biochim Biophys Acta. 1974 Jul 11;353(3):283–291. doi: 10.1016/0005-2787(74)90021-5. [DOI] [PubMed] [Google Scholar]
  17. White B. N., Tener G. M. Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus. J Mol Biol. 1973 Mar 15;74(4):635–651. doi: 10.1016/0022-2836(73)90054-5. [DOI] [PubMed] [Google Scholar]
  18. Wosnick M. A., White B. N. Purification and nucleoside composition of a Q* - containing aspartic acid tRNA from Drosophila. Biochem Biophys Res Commun. 1978 Apr 28;81(4):1131–1138. doi: 10.1016/0006-291x(78)91254-8. [DOI] [PubMed] [Google Scholar]

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