Fig. 5.

Sensitivity of P. guilliermondii wild-type strain and Δyfh1 mutant to hydrogen peroxide. Cultures of P. guilliermondii wild-type strain R-66 (WT) and Δyfh1 mutant were grown aerobically in YPD medium for 16 h, diluted to an OD₆₀₀ of 0.2 and allowed to grow for 3.5 h. Aliquots (2 ml) were treated with 1 mM H₂O₂ for 1.5 h at 30°C. Cells were pelleted at 3,000g for 10 min and re-suspended in fresh YPD medium. Suspensions were diluted 100–1,000-fold in complete medium and plated on YPD agar plates. Colonies were counted after 3 days of incubation at 30°C. Quantities of colonies obtained with untreated cultures were assumed as a 100%. Values are means ± SE of three independent experiments