CD4+CD45RBhi naïve T cells sorted from Thy1.1+ B6 congenic mice were mixed with CD4+FoxP3+ Treg cells from Thy1.2+ FoxP3-IRES-GFP reporter mice at 9:1 ratio and adoptively transferred to GITR−/−xRag−/− or Rag−/− hosts (5 mice for each group). Mice were weighed weekly and euthanized for colitis activity as described in Figure 1. Spleen, MLN and lamina propria cells from GITR−/−xRag−/− or Rag−/− recipients were isolated and evaluated for FoxP3+, Thy1.1+ and Thy1.2+ cells.
4A Weight loss as a percentage of the initial weight. Values represent the mean weight ± SD.
4B DAI. Mean and individual values of each group are indicated.
4C Ratio of Thy1.1+ and Thy1.2+ cells after cell transfer. Thy1.1+ cells represent CD4+CD45RBhi naïve T cells. Thy1.2+ cells represent FoxP3+(GFP+) cells.
4D Percentage of FoxP3(GFP)+/Thy1.2+ cells after cell transfer.
4E Percentage of FoxP3+ cells expressing Thy1.1+ and Thy1.2+. Splenocytes from GITR−/−xRag−/− or Rag−/− recipients were stained with CD4, Thy1.1 and FoxP3. Thy1.1 and FoxP3 expressing cells were compared among gated CD4+ T cells.