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. 1975 Feb;2(2):211–221. doi: 10.1093/nar/2.2.211

Study of the role of the acceptor stem in the interactions between tRNAs and aminoacyl-tRNA synthetases.

J Bonnet, N Befort, C Bollack, F Fasiolo, J P Ebel
PMCID: PMC342827  PMID: 1091915

Abstract

Several studies have clearly demonstrated that the end of the acceptor stem was a very important area determining the aminoacylation properties of tRNAs. However the attempts to measure the contribution of this region to the binding of tRNAs to aminoacyl-tRNA synthetases have led to contradictory results. We report here the stepwise degradation of yeast tRNA-Phe and tRNA-Val from their 3' terminus, up to the seventh nucleotide : the affinity of each of the degraded-tRNA for their cognate aminoacyl-tRNA synthetase was compared to that of intact tRNA and it was found that these affinities are not significantly decreased when compared to those of the intact tRNAs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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