Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1975 Feb;2(2):239–255. doi: 10.1093/nar/2.2.239

In vivo and in vitro effects of rifampicin and streptolydigin on transcription of Kluyveromyces lactis in the presence of nystatin.

P Plevani, G Badaracco, N Marmiroli, G Cassani
PMCID: PMC342829  PMID: 1091916

Abstract

Rifampicin and streptolydigin, if used in conjunction with nystatin, depress the growth of Kluyveromyces lactis. The incorporation of labeled leucine into protein is inhibited by nystatin whereas the incorporation of labeled uracil into RNA is inhibited by rifampicin in nystatin-treated cells. In order to study the mechanism of inhibition of RNA synthesis we purified by DEAE-Sephadex column chromatography four forms of RNA polymerase from K.lactis cells. The general properties of these enyzmes are similar to those of Saccharomyces cerevisiae and of other eukaryotic RNA polymerases. In particular, enzymes IA, IB and III are more active with poly[d(A-T)] template and Mn-2+ than with native or denatured calf thymus DNA. Enzyme II shows optimal activity with denatured calf thymus DNA and Mn2+. When challenged with native calf thymus DNA all enzymes prefer Mg-2+ as a divalent cation whereas with denatured calf thymus DNA all enzymes are more active with Mn-2+. Enzyme II is inhibited by lambda-amanitin but no enzyme is sensitive to rifampicin and streptolydigin. The inhibition of growth and uracil uptake observed when rifampicin is added to nystatin treated cells is probably not caused by a specific inhibition of transcription.

Full text

PDF
239

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adman R., Schultz L. D., Hall B. D. Transcription in yeast: separation and properties of multiple FNA polymerases. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1702–1706. doi: 10.1073/pnas.69.7.1702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cassani G., Burgess R. R., Goodman H. M., Gold L. Inhibition of RNA polymerase by streptolydigin. Nat New Biol. 1971 Apr 14;230(15):197–200. doi: 10.1038/newbio230197a0. [DOI] [PubMed] [Google Scholar]
  3. Haworth S. R., Brown L. R. Genetic analysis of ribonucleic acid polymerase mutants of Bacillus subtilis. J Bacteriol. 1973 Apr;114(1):103–113. doi: 10.1128/jb.114.1.103-113.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Heil A., Zillig W. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription. FEBS Lett. 1970 Dec;11(3):165–168. doi: 10.1016/0014-5793(70)80519-1. [DOI] [PubMed] [Google Scholar]
  5. Horgen P. A., Griffin D. H. Specific inhibitors of the three RNA polymerases from the aquatic fungus Blastocladiella emersonii. Proc Natl Acad Sci U S A. 1971 Feb;68(2):338–341. doi: 10.1073/pnas.68.2.338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Iwakura Y., Ishihama A., Yura T. RNA polymerase mutants of Escherichia coli. Streptolydigin resistance and its relation to rifampicin resistance. Mol Gen Genet. 1973 Mar 1;121(2):181–196. doi: 10.1007/BF00277531. [DOI] [PubMed] [Google Scholar]
  7. Küntzel H., Schäfer K. P. Mitochondrial RNA polymerase from Neurospora crassa. Nat New Biol. 1971 Jun 30;231(26):265–269. doi: 10.1038/newbio231265a0. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lindell T. J., Weinberg F., Morris P. W., Roeder R. G., Rutter W. J. Specific inhibition of nuclear RNA polymerase II by alpha-amanitin. Science. 1970 Oct 23;170(3956):447–449. doi: 10.1126/science.170.3956.447. [DOI] [PubMed] [Google Scholar]
  10. Magni G E, Von Borstel R C. Different Rates of Spontaneous Mutation during Mitosis and Meiosis in Yeast. Genetics. 1962 Aug;47(8):1097–1108. doi: 10.1093/genetics/47.8.1097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Medoff G., Kobayashi G. S., Kwan C. N., Schlessinger D., Venkov P. Potentiation of rifampicin and 5-fluorocytosine as antifungal antibiotics by amphotericin B (yeast-membrane permeability-ribosomal RNA-eukaryotic cell-synergism). Proc Natl Acad Sci U S A. 1972 Jan;69(1):196–199. doi: 10.1073/pnas.69.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mukerjee H., Goldfeder A. Purification and properties of ribonucleic acid polymerase from rat liver mitochondria. Biochemistry. 1973 Dec 4;12(25):5096–5101. doi: 10.1021/bi00749a012. [DOI] [PubMed] [Google Scholar]
  13. Rao S. S., Grollman A. P. Cycloheximide resistance in yeast: a property of the 60s ribosomal subunit. Biochem Biophys Res Commun. 1967 Dec 15;29(5):696–704. doi: 10.1016/0006-291x(67)90273-2. [DOI] [PubMed] [Google Scholar]
  14. Reusser F. Streptolydigin, an inhibitor of oxidative phosphorylation in rat liver mitochondria. J Bacteriol. 1969 Dec;100(3):1335–1341. doi: 10.1128/jb.100.3.1335-1341.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schleif R. Isolation and characterization of streptolydigin resistant RNA polymerase. Nature. 1969 Sep 6;223(5210):1068–1069. doi: 10.1038/2231068a0. [DOI] [PubMed] [Google Scholar]
  16. Scragg A. H. Mitochondrial DNA-directed RNA polymerase from Saccharomyces cerevisae mitochondria. Biochem Biophys Res Commun. 1971 Nov 5;45(3):701–706. doi: 10.1016/0006-291x(71)90473-6. [DOI] [PubMed] [Google Scholar]
  17. Siddhikol C., Erbstoeszer J. W., Weisblum B. Mode of action of streptolydigin. J Bacteriol. 1969 Jul;99(1):151–155. doi: 10.1128/jb.99.1.151-155.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sippel A. E., Hartmann G. R. Rifampicin resistance of RNA polymerase in the binary complex with DNA. Eur J Biochem. 1970 Sep;16(1):152–157. doi: 10.1111/j.1432-1033.1970.tb01066.x. [DOI] [PubMed] [Google Scholar]
  19. Tsai M. J., Michaelis G., Criddle R. S. DNA-dependent RNA polymerase from yeast mitochondria. Proc Natl Acad Sci U S A. 1971 Feb;68(2):473–477. doi: 10.1073/pnas.68.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wehrli W., Staehelin M. Actions of the rifamycins. Bacteriol Rev. 1971 Sep;35(3):290–309. doi: 10.1128/br.35.3.290-309.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wu G. J., Dawid I. B. Purification and properties of mitochondrial deoxyribonucleic acid dependent ribonucleic acid polymerase from ovaries of Xenopus laevis. Biochemistry. 1972 Sep 12;11(19):3589–3595. doi: 10.1021/bi00769a015. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES