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. 1975 Feb;2(2):279–302. doi: 10.1093/nar/2.2.279

Location and characteristics of ribosomal protein binding sites in the 16S RNA of Escherichia coli.

R A Zimmermann, G A Mackie, A Muto, R A Garrett, E Ungewickell, C Ehresmann, P Stiegler, J P Ebel, P Fellner
PMCID: PMC342832  PMID: 1091919

Abstract

Specific binding sites for five proteins of the Escherichia coli 30S ribosomal subunit have been located within the 16S RNA. The sites are structurally diverse and range in size from 40 to 500 nucleotides; their functional integrity appears to depend upon both the secondary structure and conformation of the RNA molecule. Evidence is presented which indicates that additional proteins interact with the RNA at later stages of subunit assembly.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bollen A., Herzog A., Favre A., Thibault J., Gros F. Fluorescence studies on the 30 S ribosome assembly process. FEBS Lett. 1970 Nov 9;11(1):49–54. doi: 10.1016/0014-5793(70)80489-6. [DOI] [PubMed] [Google Scholar]
  2. Branlant C., Krol A., Sriwidada J., Fellner P., Crichton R. The identification of the RNA binding site for a 50 S ribosomal protein by a new technique. FEBS Lett. 1973 Sep 15;35(2):265–272. doi: 10.1016/0014-5793(73)80301-1. [DOI] [PubMed] [Google Scholar]
  3. Brownlee G. G., Sanger F. Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli. J Mol Biol. 1967 Feb 14;23(3):337–353. doi: 10.1016/s0022-2836(67)80109-8. [DOI] [PubMed] [Google Scholar]
  4. Ehresmann C., Stiegler P., Fellner P., Ebel J. P. The determination of the primary structure of the 16S ribosomal RNA of Escherichia coli. 2. Nucleotide sequences of products from partial enzymatic hydrolysis. Biochimie. 1972;54(7):901–967. doi: 10.1016/s0300-9084(72)80007-5. [DOI] [PubMed] [Google Scholar]
  5. Ehresmann C., Stiegler P., Mackie G. A., Zimmermann R. A., Ebel J. P., Fellner P. Primary sequence of the 16S ribosomal RNA of Escherichia coli. Nucleic Acids Res. 1975 Feb;2(2):265–278. doi: 10.1093/nar/2.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fellner P., Ehresmann C., Ebel J. P. The determination of the primary structure of the 16S ribosomal RNA of Escherichia coli. 1. Nucleotide sequence analysis of T 1 and pancreatic ribonuclease digestion products. Biochimie. 1972;54(7):853–900. doi: 10.1016/s0300-9084(72)80006-3. [DOI] [PubMed] [Google Scholar]
  7. Garrett R. A., Rak K. H., Daya L., Stöffler G. Ribosomal proteins. XXIX. Specific protein binding sites on 16S rRNA of Escherichia coli. Mol Gen Genet. 1972;114(2):112–124. doi: 10.1007/BF00332782. [DOI] [PubMed] [Google Scholar]
  8. Held W. A., Ballou B., Mizushima S., Nomura M. Assembly mapping of 30 S ribosomal proteins from Escherichia coli. Further studies. J Biol Chem. 1974 May 25;249(10):3103–3111. [PubMed] [Google Scholar]
  9. Mizushima S., Nomura M. Assembly mapping of 30S ribosomal proteins from E. coli. Nature. 1970 Jun 27;226(5252):1214–1214. doi: 10.1038/2261214a0. [DOI] [PubMed] [Google Scholar]
  10. Muto A., Ehresmann C., Fellner P., Zimmermann R. A. RNA-protein interactions in the ribosome. I. Characterization and ribonuclease digestion of 16 S RNA-ribosomal protein complexes. J Mol Biol. 1974 Jun 25;86(2):411–432. doi: 10.1016/0022-2836(74)90028-x. [DOI] [PubMed] [Google Scholar]
  11. Noller H. F. Topography of 16S RNA in 30S ribosomal subunits. Nucleotide sequences and location of sites of reaction with kethoxal. Biochemistry. 1974 Nov 5;13(23):4694–4703. doi: 10.1021/bi00720a003. [DOI] [PubMed] [Google Scholar]
  12. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  13. Schaup H. W., Green M., Kurland C. G. Molecular interactions of ribosomal components. I. Identification of RNA binding sites for individual 30S ribosomal proteins. Mol Gen Genet. 1970;109(3):193–205. doi: 10.1007/BF00267007. [DOI] [PubMed] [Google Scholar]
  14. Schaup H. W., Green M., Kurland C. G. Molecular interactions of ribosomal components. II. Site-specific complex formation between 30S proteins and ribosomal RNA. Mol Gen Genet. 1971;112(1):1–8. doi: 10.1007/BF00266926. [DOI] [PubMed] [Google Scholar]
  15. Schaup H. W., Kurland C. G. Molecular interactions of ribosomal components. 3. Isolation of the RNA binding site for a ribosomal protein. Mol Gen Genet. 1972;114(4):350–357. doi: 10.1007/BF00267503. [DOI] [PubMed] [Google Scholar]
  16. Schaup H. W., Sogin M. L., Kurland C. G., Woese C. R. Localization of a binding site for ribosomal protein S8 within the 16S ribosomal ribonucleic acid of Escherichia coli. J Bacteriol. 1973 Jul;115(1):82–87. doi: 10.1128/jb.115.1.82-87.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schaup H. W., Sogin M., Woese C. Characterization of an RNA "binding site" for a specific ribosomal protein of Escherichia coli. Mol Gen Genet. 1972;114(1):1–8. doi: 10.1007/BF00268740. [DOI] [PubMed] [Google Scholar]
  18. Schulte C., Morrison C. A., Garrett R. A. Protein-ribonucleic acid interactions in Escherichia coli ribosomes. Solution studies on S4-16S ribonucleic acid and L24-23S ribonucleic acid binding. Biochemistry. 1974 Feb 26;13(5):1032–1037. doi: 10.1021/bi00702a031. [DOI] [PubMed] [Google Scholar]
  19. Székely M., Brimacombe R., Morgan J. A specific ribonucleoprotein fragment from Escherichia coli 30-S ribosomes. Location of the RNA component in 16-S RNA. Eur J Biochem. 1973 Jun 15;35(3):574–581. doi: 10.1111/j.1432-1033.1973.tb02875.x. [DOI] [PubMed] [Google Scholar]
  20. Zimmermann R. A., Muto A., Fellner P., Ehresmann C., Branlant C. Location of ribosomal protein binding sites on 16S ribosomal RNA. Proc Natl Acad Sci U S A. 1972 May;69(5):1282–1286. doi: 10.1073/pnas.69.5.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zimmermann R. A., Muto A., Mackie G. A. RNA-protein interactions in the ribosome. II. Binding of ribosomal proteins to isolated fragments of the 16 S RNA. J Mol Biol. 1974 Jun 25;86(2):433–450. doi: 10.1016/0022-2836(74)90029-1. [DOI] [PubMed] [Google Scholar]

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