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. 1975 Mar;2(3):361–371. doi: 10.1093/nar/2.3.361

Photo-induced cross-linkage of gene-5 protein and bacteriophage fd DNA+

Eric Anderson 1, Yasutsuga Nakashima 1, William Konigsberg 1
PMCID: PMC342842  PMID: 1093139

Abstract

The gene 5 protein, coded for by the bacteriophage fd, forms a complex with single stranded fd-DNA such that one gene 5 protein monomer interacts with four bases. Exposure of this complex to ultraviolet light results in the formation of covalent bonds between 25-30% of the gene 5 protein monomers which are bound to the DNA. In contrast, when the intact fd virion, which is a complex of coat protein and DNA, was exposed to ultraviolet irradiation, no detectable protein DNA cross-links were found.

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Selected References

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